For medium- to high-throughput sample disruption for molecular analysis
Convenient and secure disruption process
Adapter sets optimized for high-throughput disruption
Wide range of accessories available
Reproducible results with difficult-to-lyse tissues
Front-end solution for QIAGEN automation
The TissueLyser II simultaneously disrupts multiple biological samples through high-speed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads. Using the appropriate adapter set, up to 48 or 192 samples can be processed at the same time. Alternatively, a grinding jar set can be used to process large samples. A range of beads, bead dispensers, and collection microtubes and caps are also available. All accessories for the TissueLyser II, including adapter sets, are also compatible with the TissueLyser (no longer available).
Bead mill, 100–120/220–240 V, 50/60 Hz; requires the TissueLyser Adapter Set 2 x 24 or TissueLyser Adapter Set 2 x 96 (available separately)
The TissueLyser II is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Reliable detection of miRNAs.
Rat kidney was stabilized in RNAlater RNA Stabilization Reagent, and 25 mg samples were disrupted in QIAzol Lysis Reagent for 2 x 5 minutes at 25 Hz using the TissueLyser II. Total RNA containing miRNA was purified using the miRNeasy Mini Kit, using either the manual procedure (Manual) or the automated procedure with the QIAcube (QIAcube). Real-time RT-PCR using the miScript System was carried out to detect 2 different miRNAs, miR-16 and miR-25.
Efficient disruption of animal tissues.
Tissues (20 mg) were either frozen (Frozen) or stabilized in RNAlater RNA Stabilization Reagent (RNAlater), and then disrupted using the TissueLyser II (2 x 2 minutes for liver and muscle; 2 x 5 minutes for skin). RNA was purified using the RNeasy Mini Kit (liver), RNeasy Fibrous Tissue Mini Kit (skin), or RNeasy Lipid Tissue Mini Kit (muscle). [A] Analysis on a 1.2 % formaldehyde agarose gel shows sharp ribosomal RNA bands, indicating intact RNA. [B] RNA yields were quantified by A260 nm absorbance measurements.
Reproducible RNA purification from plant tissues.
Frozen plant leaves were disrupted using the TissueLyser II (2 x 1 minute). RNA was purified using the RNeasy Plant Mini Kit and analyzed on a 1.2 % formaldehyde agarose gel. The ribosomal RNA bands were sharp and of equal intensity, indicating reproducible purification of intact RNA. T: tomato (100 mg); A: arabidopsis (25 mg); C: cotton (100 mg); M: maize (100 mg); R: rape (100 mg).
Intact protein suitable for all types of analysis.
Various rat tissues (25 mg each)
stabilized in Allprotect Tissue Reagent were disrupted using the TissueLyser LT or TissueLyser II.
Total protein was purified using the Qproteome Mammalian Protein Prep Kit and then
analyzed by western blotting. Transfer membrane stained with Ponceau S, and western
blot. LT: TissueLyser LT; II: TissueLyser II; M: markers.
High-quality DNA from animal tissues.
Various rat
tissues (25 mg each) stabilized in Allprotect Tissue Reagent
were disrupted using the TissueLyser LT or TissueLyser II.
DNA was purified on the QIAcube using the DNeasy Blood
& Tissue Kit, and then used in PCR with the HotStarTaq Plus
Master Mix Kit and a PGK1 primer system. The 120 bp PCR
product was analyzed on the QIAxcel using the QIAxcel
DNA High Resolution Kit. LT: TissueLyser LT; II: TissueLyser II;
M: markers.
High-quality DNA from plant tissues.
Various
plant tissues (100 mg each) were disrupted in precooled
adapters using the TissueLyser LT or TissueLyser II. DNA was
purified on on the QIAcube using the DNeasy Plant Mini Kit
and then analyzed on an agarose gel. LT: TissueLyser LT;
II: TissueLyser II; M: markers.
Genotyping, gene expression, and proteomics applications demand effective disruption
of biological samples to ensure high yields of DNA, RNA, and protein. Effortless
disruption with the TissueLyser II system is achieved through high-speed shaking
with beads, which beat and grind samples. The TissueLyser II system delivers thorough
and rapid disruption of samples to fully release biomolecules, and also simultaneously
homogenizes samples to facilitate subsequent purification procedures using QIAGEN
kits (see table "QIAGEN purification kits compatible with QIAGEN disruption systems").
The TissueLyser II is an integral part of QIAGEN's complete solution for sample
management — from sample collection to purification and analysis of DNA, RNA, and
protein. The TissueLyser II complements QIAGEN automation for high-throughput sample
preparation and analysis (see table "QIAGEN high-throughput automation"), such as
the QIAsymphony SP. This easy-to-use instrument automates purification of DNA, RNA,
and protein from 1–96 samples. The TissueLyser II is also compatible with QIAGEN
manual sample preparation kits (see table "QIAGEN purification kits compatible with
QIAGEN disruption systems").
For RNA applications, stabilization of fresh tissues in RNAlater RNA Stabilization
Reagent prevents RNA degradation during sample handling. For applications requiring
purification of DNA, RNA, and protein, these 3 analytes can be immediately stabilized
by placing fresh tissues in Allprotect Tissue Reagent.
QIAGEN high-throughput automation
Instrument
Purpose
Throughput
QIAsymphony SP
Purification of DNA, RNA, and protein
1–96 samples per run
BioRobot Universal System
Purification of DNA and RNA
96-well format
QIAxcel
Electophoretic analysis of DNA fragments and RNA
Up to 96 samples per run
QIAgility
Reaction setup
Up to 384 samples per run
Rotor-Gene Q
Real-time PCR and high-resolution melting (HRM) analyses
Up to 100 samples per run
PyroMark Q96 MD or ID
Methylation and mutation analyses
Up to 96 samples per run
QIAGEN purification kits compatible with QIAGEN disruption systems
Analyte purified
Sample type
QIAGEN kit
RNA
Easy-to-lyse tissue
RNeasy Kits
RNeasy Plus Kits
RNeasy Protect Kits
RNA
Fiber-rich tissue
RNeasy Fibrous Tissue Kits
RNA
All types of tissue
RNeasy Lipid Tissue Kits
RNeasy Universal Tissue Kits
RNA
Plant tissue
RNeasy Plant Mini Kit
RNA
Yeast
RNeasy Kits
RNA
Bacteria
RNeasy Protect Bacteria Kits
microRNA
All types of tissue
miRNeasy Kits
DNA
Human tissue
QIAamp DNA Kits
DNA
Animal tissue
DNeasy Blood & Tissue Kits
Protein
Tissue
Qproteome Mammalian Protein Prep Kit
Phosphoprotein
Tissue
PhosphoProtein Purification Kit
Glycoprotein
Tissue
Qproteome Glycoprotein Kits
DNA and RNA
Tissue
AllPrep DNA/RNA Kits
DNA, RNA, and protein
Tissue
AllPrep DNA/RNA/Protein Mini Kit
Procedure
TissueLyser Adapter Sets allow processing of up to 2 x 96 samples in collection microtubes or up to 2 x 24 samples in 2 ml microcentrifuge tubes. The adapter sets can be precooled at –80°C if disrupting samples without lysis buffer. Tubes remain securely sealed during disruption to prevent cross-contamination, which is especially important for highly sensitive downstream applications, such as real-time RT-PCR or microarray analysis. Depending on the sample type, disruption is carried out using stainless steel, tungsten carbide, or glass beads. TissueLyser Bead Dispensers are available to conveniently deliver single beads into microcentrifuge tubes or to deliver 96 beads in parallel into collection microtubes. The TissueLyser II can also disrupt large samples when used in combination with Grinding Jar Sets.
Applications
The ability to process up to 192 samples per run makes the TissueLyser II the ideal front-end solution to access biological information for genomics, transcriptomics, and proteomics applications. For next-generation, high-throughput sequencing technologies, the TissueLyser II is the disruption instrument of choice. A wide range of QIAGEN sample purification kits are compatible with the TissueLyser II, and sample purification can be performed manually or can be automated using the QIAcube, QIAsymphony SP, EZ1 Advanced, or BioRobot or BioSprint workstations.
Features
Specifications
Disruption principle
High-speed shaking of samples in 1.2 ml collection tubes or 2 ml microcentrifuge tubes with stainless steel or glass beads
Features
Convenient and secure disruption process. Adapter sets optimized for high-throughput disruption. Wide range of accessories available (e.g.,grinding jar set to process large samples). Reproducible results with difficult-to-lyse tissues. Front-end solution for QIAGEN automation.
Kits compatible with instrument
All kits for purifying RNA/DNA/Protein
Protocol/main application on this intrument
Sample preparation/sample disruption
Technical data
100–120/220–240 V, 50/60Hz; variable speeds from 3 to 30 Hz (180–1800 oscillations/minute)
Technology
Bead Mill
Throughput
2 x 96 collection microtubes (1.2 ml) or 2 x 24 microcentrifuge tubes (2ml)
