Cat. No. / ID: 9002340
QIAxpert exhibits excellent linearity and assures accurate and reliable quantifications. To assess the linearity of the system, pure gDNA was serially diluted to generate samples from 2000 to 2 ng (see figure Exceptional linearity of QIAxpert). The resulting dilutions were then measured on the QIAxpert using 2 µl for each of 5 replicates. The quantifications from the QIAxpert were plotted against the known concentration values, and the resulting plot demonstrates the exceptional linearity (with a regression coefficient of >0.999) of QIAxpert up to 2000 ng/ml or 40 OD (A260, 10 mm path length).
To demonstrate the reproducibility of measurements with the QIAxpert, 10 ultrapure dsDNA samples were repeatedly quantified with the instrument. The sample concentrations spanned the complete dynamic range of the system and each sample was measured 10 times. Standard deviation (SD) and coefficient of variation (CV) were calculated to illustrate the minimal variability from sample to sample. Results from 6 representative samples are shown (see table below).
|Sample 1||Sample 2||Sample 3||Sample 4||Sample 5||Sample 6|
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|Type||UV/VIS polychromatic system + reference channel|
|Wavelength resolution||<3 nm|
|Dimensions||(W) 23 x (D) 28 x (H) 30 cm; 9 kg|
|Wavelength reproducibility||0.1 nm|
|Light source||Xenon flash lamp|
|Wavelength range||230–750 nm|
|Wavelength accuracy||≤0.2 nm|
|PowerSupply||100–240 V, 50/60Hz (operating voltage 24 VDC)|
|Photometric range||0.0005–2.0 OD|
|Samples per run (throughput)||1–16 samples per run (blanks included)|
|Data output||USB, TCP/IP or QR code|
|Software||On-board software; newest software can be downloaded at www.qiagen.com/QIAxpert|
|Measurement time||Approximately 2 minutes for fully loaded slide|
|Maximum sample residence time||2 hours|
|Recommended sample quantity||2 µl|
|Measurement range (10 mm equivalent)||0.03–40 OD|
|Concentration range||1.5 ng/μl up to 2000 ng/μl dsDNA (A260 dsDNA application)|
|Optical principle||Ultrasensitive photodiode array spectrophotometer for UV-VIS range|
|Absorbance accuracy||4% (0.66 OD at 350 nm)|
|Absorbance precision||0.001 OD|
|Path length||0.5 mm|