Investigator Lyse&Spin Basket Kit

For lysis and filtration of forensic samples to remove solid sample substrates

Features

  • Perform lysis and filtration of forensic samples on solid substrates
  • Can be used for a variety of sample types
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Investigator Lyse&Spin Basket Kit (250)

Cat. No. / ID: 19598

10 pouches containing 5 x 50 baskets and 5 x 50 collection tubes
£176.00
Preparations
250
50
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The Investigator Lyse&Spin Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details


The Investigator Lyse&Spin Basket Kit is used to perform lysis and filtration of forensic samples on solid substrates. The procedure can be used for swabs, pieces of fabric or leather, paper, cigarette butts, chewing gum, small tapes, or other sample types.

Principle

Lyse&Spin baskets are used for pretreatment of forensic samples in combination with manual and automated extraction kits, for example, EZ1 DNA Investigator, QIAsymphony DNA Investigator, or QIAamp DNA Investigator Kits. They permit the combination of sample lysis and separation of solid sample substrates in one simple procedure. No sample transfer is required to obtain a cleared lysate.

Procedure

The basket retains the lysis buffer during the lysis step of the forensic sample. Upon centrifugation on an ordinary bench top centrifugation system, holes in the bottom of the basket open up and let the sample lysate pass through the basket. The lysate, which contains nucleic acids, is efficiently recovered and collected in the provided 2 ml collection tube, while the solid particles remain in the basket. Used baskets with solid sample substrates can optionally be stored in the 2nd collection tube provided.

Applications

The Investigator Lyse&Spin Basket Kit allows the combination of sample lysis and separation of solid sample substrates in forensics and genetic identity testing, for example, buccal or surface swabs, fabrics, cigarette butt paper, and others.

Resources

Brochures & Guides (2)
Advanced sample collection, automation and STR solutions for kinship testing
Kit Handbooks (1)
Application Notes (2)

High-throughput DNA sample prep using the QIAsymphony SP instrument: An overview of workflow optimization at Bode Cellmark Forensics.

Supplementary Protocols (2)
Supplementary protocol using the EZ1 Advanced or EZ1 Advanced XL with the EZ1 DNA Investigator Kit
Supplementary protocol using the Investigator Lyse&Spin Basket Kit and EZ1 Advanced XL
Technical Information (2)
Quality initiatives for human identity testing and forensics 

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699