The QIAseq FX Single Cell DNA Library Kit is a complete cell-to-library solution that includes all of the reagents necessary to generate high-diversity whole genome libraries from single eukaryotic or bacterial cells or from limited amounts of intact gDNA. The kit contains reagents for the effective cell lysis of isolated single cells or small clusters of cells, for example from laser-capture microdissection platforms. After lysis, genomic DNA is amplified using an ultra-high fidelity multiple displacement annealing technology. Using viable cells as input, this procedure generates large amounts of amplified gDNA. This amplified gDNA is subjected to a random and efficient enzymatic fragmentation method, which cleaves the DNA into shorter fragments compatible with any Illumina NGS instrument. These fragments are then end-polished and subjected to a highly efficient ligation reaction with the included, single-use adapters. This effectively captures the fragmented DNA into a library, which can be purified using standard methods prior to sequencing. Additional PCR amplification of the library is unnecessary, and the entire procedure is PCR-free, eliminating the possibility of introducing PCR duplicates and maximizing library diversity.