For control experiments when using Cignal Reporter Assays
Cat. No. / ID: 336881
Cignal Reporter Assays provide a rapid, sensitive, and quantitative assessment of signal transduction pathway activation by measuring the activities of downstream transcription factors. When conducting experiments using Cignal Reporter Assays, proper controls are a key element of the experimental design. They permit accurate interpretation of reporter assay results and provide assurance of the specificity of the observed response.
Cignal Reporter Controls are preformulated, transfection-ready constructs. Positive and negative controls are available for both dual-luciferase and GFP reporters.
The following controls are available.
| Control | Description |
|---|---|
| Positive Control Assay (GFP) | Constitutively expressing GFP gene. Easily measure transduction efficiency and optimize transduction conditions with green fluorescent protein (GFP) |
| Negative Control Assay (GFP) | Noninducible, minimal promoter-driven GFP gene. Establish the specificity of any treatment effects and determine background GFP fluorescence |
| Positive Control Assay (luc) | 40:1:1 mixture of constitutively expressing GFP, constitutively expressing firefly luciferase, and constitutively expressing Renilla luciferase constructs. Measure transduction efficiency and serve as positive control for firefly luciferase assay |
| Negative Control Assay (luc) | 40:1 mixture of non-inducible firefly luciferase construct and constitutively expressing Renilla luciferase construct. Establish the specificity of any treatment effects |
Cignal Lenti Reporter Control Assays are pretitered, transduction-ready lentivirus particles used for optimizing transduction conditions. Use standard procedures to transduce Cignal Lenti Reporter Control Assays into selected cells, and treat control-transduced cells in the same way as reporter-transduced cells.
Cignal Reporter Controls are a key element of the experimental design when conducting experiments using Cignal Reporter Assays. They are versatile tools for:
