PyroMark Custom Assays give users the flexibility to order a high-quality Pyrosequencing kit for the analysis of any DNA sequence. Enter the sequence of pre-designed PCR and sequencing primers, and receive a ready-to-use assay with optimized primer concentrations. The source of the primer sequences you enter may be from the literature, from previous projects, or — to maximize analysis success — from primer design and evaluation using the PyroMark Assay Design Software 2.0. Combined with other dedicated products of the PyroMark line, including carefully formulated PCR and sequencing reagents, the PyroMark Custom Assays expand the streamlined Pyrosequencing workflow to enable analysis of virtually any sequence in any genome.
Optimized primer concentration
Both the PCR and sequencing primers included in PyroMark Custom Assays are delivered at optimal concentrations for convenience of use and enhanced PCR quality. Together with the carefully formulated reagents of the PyroMark PCR Kit, this optimization ensures improved yield and quality of sequencing template, which consequently increases assay reliability. If working with RNA samples, the new PyroMark OneStep RT-PCR Kit enables reverse transcription and template amplification in one reaction with the same optimized yield and assay reliability.
The advantages of GeneGlobe
QIAGEN's GeneGlobe Web portal is a one-stop online interface that grants access to our extensive range of assay technologies in a gene- or pathway-specific context. GeneGlobe brings together state-of-the-art search and design algorithms, up-to-date sequence data, and our innovative technologies to facilitate discovery. Simply enter a search term for the gene or pathway of interest, and let GeneGlobe show you how to advance your research in epigenetics, gene expression, genetic determinants of disease, and more (see figure " Ordering through GeneGlobe").