text.skipToContent text.skipToNavigation

RNeasy PowerBiofilm Kit

For the isolation of high quality total RNA from biofilm samples
  • High-quality RNA isolation from all biofilms, challenging dental plaques and microbial mats
  • High RNA yields with pre-treatment, mechanical and chemical lysis
  • Efficient elimination of PCR inhibitors with Inhibitor Removal Technology
Overcome inefficient cell lysis with the RNeasy PowerBiofilm Kit. Mechanical and chemical lysis are combined with Inhibitor Removal Technology to isolate pure RNA free from inhibitors typical of biofilms, including humic acid, metals, salts and pesticides. The resulting RNA is then ready for further analysis with downstream applications.

The RNeasy PowerBiofilm Kit was formerly sold by MO BIO as PowerBiofilm RNA Isolation Kit (50).
Cat No./ID: 25000-50
RNeasy PowerBiofilm Kit (50)
611,00 €
Order Product
For the isolation of high quality total RNA from biofilm samples
The RNeasy PowerBiofilm Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Le site web n’est pas disponible en français dans son intégralité. Son contenu est international et peut aller à l’encontre des règlementations locales.
0
Figure 1. High-quality RNA yield from a range of biofilms.
High-quality and yield RNA was recovered from samples regardless of biofilm origin. Several types of biofilms were collected and RNA isolated from those samples. Resulting RNA quality was assessed through gel electrophoresis; RNA yield was determined using NanoDrop.

*All yields represent combined results from multiple experiments and collaborators.
1
Figure 2. Improved success of downstream applications.
Successful reverse transcription of RNA performed using the QuantiTech® Reverse Transcription Kit. Universal 16S rRNA gene (StreF/StreR) primers were used. This generated a 1,243 bp product. PCR was run using the KAPATM Fast HotStart Kit.
2
Figure 3. More positive results.
The E. coli 6-point standard curve is represented by grey curves. Reverse transcribed RNA isolated from lagoon rock biofilms are represented by blue curves.
Performance


Features
Specifications
Bead size 0.15 mm garnet
Binding capacity Up to 40 µg per prep
Format Silica Spin Filter Tubes
Processing Bead beating
Sample size 0.05 - 0.20 g
Sample types All biofilms from cultured single cell layers to microbial mats
Storage temperature Store lyophilized DNase I at 4°C All other kit reagents and components should be stored at room temperature (15-30°C)
Throughput 1-24 samples
Time per run or per prep 42 minutes (+15 minute DNase step)
Le site web n’est pas disponible en français dans son intégralité. Son contenu est international et peut aller à l’encontre des règlementations locales.
fragment fix placeholder
Le site web n’est pas disponible en français dans son intégralité. Son contenu est international et peut aller à l’encontre des règlementations locales.

Recommended for you

  • Cat No./ID: 331505
    /fr/shop//sequencing/qiaseq-mirna-ngs/

    QIAseq miRNA Library Kit (96)

    For 96 sequencing prep reactions: 3’ ligation, 5’ ligation, reverse-transcription, cDNA cleanup, library amplification and library cleanup reagents; quality control primers
    5 910,00 €
  • Cat No./ID: 208354
    /fr/shop//pcr/quantinova-probe-rt-pcr-kit/

    QuantiNova Probe RT-PCR Kit (500)

    For 500 x 20 µl reactions: 3 x 1.7 ml QuantiNova Probe RT-PCR Master Mix, 100 µl QuantiNova Probe RT Mix, 100 µl Internal Control RNA, 500 µl Yellow Template Dilution Buffer, 1 ml ROX Reference Dye, 2 x 1.9 µl RNase-Free Water
    646,00 €
  • Cat No./ID: 208552
    /fr/shop//pcr/quantinova-multiplex-rt-pcr-kit/

    QuantiNova Multiplex RT-PCR Kit (100)

    For 100 x 20 µl reactions: 0.5 ml 4x QuantiNova Multiplex RT-PCR Master Mix, 20 µl QuantiNova Multiplex RT-Mix, 20 µl QuantiNova IC RNA, 500 µl QuantiNova Yellow Template Dilution Buffer, 250 µl QN ROX Reference Dye, 1.9 ml RNase-Free Water
    179,00 €