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Allprep PowerViral DNA/RNA Kit

For isolating viral or bacterial total nucleic acids from waste water and stool samples

  • Optimized to isolate viral or bacterial DNA and RNA from waste water, stool, biosolids and gut material
  • Inhibitor Removal Technology eliminates inhibitory substances, including polysaccharides, lipids and heme for nucleic acids that are ready to use in PCR, qPCR, RT-qPCR, RNA-Seq, Sanger sequencing or NGS
  • Optional bead beating protoocol available with Glass Bead Tubes for optimal lysis of solid samples and bacterial cells and without bead tubes for isolation of viral nucleic acids from liquid samples

The Allprep PowerViral DNA/RNA Kit is designed for fast and easy purification of viral and bacterial total nucleic acids from samples high in PCR inhibitors; including waste water, stool, biosolids, and gut material. Patented Inhibitor Removal Technology (IRT) ensures complete removal of the inhibitory substances often contained in these materials, such as undigested plant material in the gut or heme compounds from lysed red blood cells, abundant in stool. The result is DNA and RNA from virus and bacteria that is ready to use in the most demanding downstream applications. Nucleic acids are eluted in RNase-Free Water and ready for PCR, cDNA synthesis, RT-qPCR, RNA-seq, Sanger sequencing or NGS.

For stool samples or samples with a solid matrix, lysis is achieved using 0.1 mm Glass Bead Tubes in combination with a strong chemical lysis buffer that ensures efficient extraction of tough microorganisms in the bead beating step. For virus extraction from water samples and samples that do not require dispersion, the bead beating step may be skipped and virus extracted through chemical lysis only.

The Allprep PowerViral DNA/RNA Kit was previously sold by MO BIO as the PowerViral Environmental DNA/RNA Kit.

Cat No./ID: 28000-50
AllPrep PowerViral DNA/RNA Kit (50)
500,00 €
Order Product
For isolating viral or bacterial total nucleic acids from waste water and stool samples
The Allprep PowerViral DNA/RNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Le site web n’est pas disponible en français dans son intégralité. Son contenu est international et peut aller à l’encontre des règlementations locales.
Performance



Features
Specifications
Bead size 0.1 mm glass (optional)
Binding capacity Up to 40 µg per spin filter
Format Silica Spin Filter Columns
Processing Chemical lysis and/or bead beating
Sample size 200 µl or 0.25 g
Sample types Processed waste water, stool, biosolids and gut material
Storage temperature Store at room temperature (15-30°C)
Throughput 1-24 samples
Time per run or per prep 45 minutes
Le site web n’est pas disponible en français dans son intégralité. Son contenu est international et peut aller à l’encontre des règlementations locales.
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Le site web n’est pas disponible en français dans son intégralité. Son contenu est international et peut aller à l’encontre des règlementations locales.

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