Highly effective lysis of solid and liquid samples with bead beating protocol
The AllPrep PowerViral DNA/RNA Kit is designed for fast and easy purification of viral and bacterial total nucleic acids even from samples high in PCR inhibitors; including waste water, stool, biosolids and gut material. Inhibitor Removal Technology ensures complete removal of inhibitory substances often contained in these materials, such as undigested plant material in the gut or heme compounds from lysed red blood cells that are abundant in stool. The result is DNA and RNA from virus and bacteria that is ready to use in the most demanding downstream applications. Nucleic acids are eluted in RNase-free water and ready to use in PCR, cDNA synthesis, RT-qPCR, RNA-seq, Sanger sequencing or next-generation sequencing.
For stool samples or samples with a solid matrix, lysis is achieved using 0.1 mm Glass Bead Tubes in combination with a strong chemical lysis buffer that ensures efficient extraction of tough microorganisms in the bead beating step. For virus extraction from water samples and samples that do not require dispersion, the bead beating step may be skipped and virus extracted through chemical lysis only.
Buffers and reagents for DNA fragmentation (including end repair and A-addition), ligation and library amplification; for use with Illumina instruments; includes a plate containing 96 adapters with different barcodes (pierceable foil seal, allowing usage of defined parts of plate)