MinElute Reaction Cleanup Kit

For cleanup of up to 5 μg DNA (70 bp to 4 kb) from enzymatic reactions
  • Very small elution volumes
  • Fast procedure and easy handling
  • High, reproducible recoveries
  • Gel loading dye for convenient sample analysis

The MinElute Reaction Cleanup Kit provides spin columns, buffers, and collection tubes for silica membrane-based purification of DNA 70 bp – 4 kb in size from enzymatic reactions. The spin columns are designed to allow elution in very small volumes (as little as 10 μl), delivering highly concentrated DNA in high yields. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube Connect. DNA fragments purified with the MinElute system are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.

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製品名 Cat. no. List price:
MinElute Reaction Cleanup Kit (50)
50 MinElute Spin Columns, Buffers, Collection Tubes (2&nbsp:ml)
28204
¥16,000
MinElute Reaction Cleanup Kit (250)
250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
28206
¥65,000

MinElute Reaction Cleanup Kit  は分子生物学実験用です。疾病の診断、治療または予防の目的には使用することはできません。


  • Main Image Navi
MinElute procedure.|GelPilot Loading Dye.|MinElute membrane assembly.|pH Indicator Dye.|Spin column handling options — D.|Spin column handling options — E.|Spin column handling options — C.|Spin column handling options — B.|Spin column handling options — A.|
This simple bind–wash–elute procedure ensures greater convenience.


|GelPilot Loading Dye contains three tracking dyes to facilitate optimization of DNA resolution.

|MinElute spin column in cross section, showing the unique membrane assembly (utility model pending).
|pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case, the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.
|QIAvac 24 plus.
|QIAcube.
|Manifold with luer connectors.
|QIAvac 24.
|Microcentrifuge.
|
Performance

The MinElute Reaction Cleanup Kit ensures cleanup of up to 5 μg DNA (70 bp to 4 kb) from enzymatic reactions, delivering high yields of DNA suitable for a range of applications. The kit provides spin columns for cleanup of enzymatic reactions. Using a microcentrifuge or vacuum manifold, high concentration of DNA fragment (70 bp – 4 kb) is quickly achieved. (DNA fragments larger than 4 kb should be purified using the QIAquick System.)

Examples of enzymes that are completely removed by the MinElute Reaction Cleanup Kit
ProteinMolecular weight per enzyme subunit (kDa)
DNA Polymerase I 109
Klenow fragment 62
Calf intestinal alkaline phosphatase 69
T4 DNA ligase 55
T4 Polynucleotide kinase 35
Terminal transferase 32
DNase I 31
Restriction enzymes Varies
Principe

MinElute Kits contain a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples. Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges.

Gel loading dye

To enable faster and more convenient sample processing and analysis, gel loading dye is provided. GelPilot Loading Dye contains three tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far (see figure "GelPilot Loading Dye").

Procédure

The MinElute system uses a simple bind-wash-elute procedure (see flowchart "MinElute procedure"). Binding buffer is added directly to the enzymatic reaction, and the mixture is applied to the MinElute spin column. The binding buffer contains a pH indicator, allowing easy determination of the optimal pH for DNA binding (see figure "pH Indicator Dye"). Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Handling

MinElute spin columns are designed to provide two convenient handling options (see flowchart "MinElute procedure"). The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as QIAvac 24 Plus or QIAvac 6S with QIAvac Luer Adapters. The MinElute Reaction Cleanup Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated on the QIAcube, enabling increased productivity and standardization of results (see figures "Spin column handling options A, B, C, D, and E").

Applications

DNA fragments purified with the MinElute System are ready for direct use in all applications, including:

Sequencing
Microarray analysis
Ligation and transformation
Restriction digestion
Labeling
Feature
Specifications
Binding capacity 5 µg
Elution volume 10 µl
Format Tube
Fragment size 70 bp – 4 kb
Processing Manual
Recovery: oligonucleotides dsDNA Recovery: oligonucleotides, dsDNA
Removal <10mers 17–40mers dye terminator proteins Removal <40mers
Sample type: applications DNA, oligonucleotides: Enzymatic reactions
Technology Silica technology

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FAQ
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キットハンドブック
2
MinElute PCR Purification Kit
低溶出量でのPCR産物(70 bp~ 4 kb)精製用

MinElute Gel Extraction Kit
低溶出量でのDNAフラグメント(70 bp~ 4 kb)のゲル抽出

MinElute Reaction Cleanup Kit
酵素反応液からのDNA(70 bp~ 4 kb)クリーンアップ用
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クイックスタートプロトコール
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MinElute Procedure
MinElute操作手順

この結合―洗浄―溶出の簡単な操作によって、簡便性が確実に高まる。


GelPilot Loading Buffer
GelPilot Loading Dye
GelPilot Loading Dye に入っている3種類のマーカー色素によりDNA電気泳動の至適化が容易。

MinElute Membrane Assembly
MinEluteメンブレン構造
ユニークなメンブレン構造を持つMinElute Spin Column断面図(utility model pending)。
ILLU_0104_MinElute
pH 指示薬
溶解および結合バッファー中のpH指示薬により、DNA吸着の至適pH(pH <7.5)かどうかを簡単に確認できる。アガロースゲル電気泳動バッファーを繰り返し使用したり、間違って調製した場合に、結合溶液のpHは至適pHより高くなる。このような場合は、3M 酢酸ナトリウム、pH 5.0 を10 µl 添加してpHを簡単に調整することが可能。
Spin Column操作オプション - D
QIAvac 24 Plus
Spin Column操作オプション - E
QIAcube
QIAprep Spin Column handling options
Spin Column操作オプション - C
ルアーコネクター付きマニホールド
QIAprep Spin Column handling options
Spin Column操作オプション - B
QIAvac 24
QIAprep Spin Column handling options
Spin Column操作オプション - A
マイクロ遠心機