mericon Vibrio triple Kit
For real-time PCR detection of Vibrio vulnificus, V. parahaemolyticus, and V. cholerae in food or animal feed
The mericon Vibrio triple Kit is designed for the target-specific detection of Vibrio vulnificus, Vibrio parahaemolyticus, and Vibrio cholerae DNA in food, animal feed, and pharmaceutical products. The assay uses a real-time PCR-based protocol and forms part of the comprehensive QIAGEN food testing portfolio, which also features dedicated sample preparation kits and assays for ingredient authentication and GMO DNA detection. The mericon Vibrio triple assay performs optimally on the Rotor-Gene Q, but has also been validated for block thermal cyclers.
The mericon Vibrio triple Kit is intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Le site web n’est pas disponible en français dans son intégralité. Son contenu est international et peut aller à l’encontre des règlementations locales.
Performance
The mericon Vibrio triple Kit is for the specific detection of Vibrio vulnificus, Vibrio parahaemolyticus, and Vibrio cholerae in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Vibrio vulnificus, Vibrio parahaemolyticus, and Vibrio cholerae DNA in a reaction and exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Vibrio triple Kit cross-reactivity study"). Results of mericon Vibrio triple Kit cross-reactivity study
Principle
The mericon Vibrio triple Kit is designed for the qualitative detection of Vibrio vulnificus, Vibrio parahaemolyticus, and Vibrio cholerae in food and animal feed samples after enrichment. All three species of Vibrio are human pathogenic and cause food-borne diseases such as gastroenteritis. Vibrio cholerae is known to be the pathogen for cholera due to secretion of the cholera toxin. Detection of pathogens using real-time PCR is based on the amplification of a specific region of the relevant pathogen genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward. mericon PCR Assays include an internal control to control potential inhibition. This aids in the straightforward interpretation of the results (see the table "Summary of possible PCR outcomes"). The PCR primer set for each assay is highly specific and targets a unique and conserved region of the tested pathogen genome that has been bioinformatically and experimentally verified.
Procedure
DNA is extracted from an enrichment culture of a sample of food or animal feed using a dedicated sample preparation kit. The mericon Bacteria Kit is for the isolation of bacterial DNA from Gram-negative bacteria. The mericon Bacteria Plus Kit is for the isolation of bacterial DNA from Gram-positive bacteria. The isolated, pure bacterial DNA is assayed using a streamlined real-time PCR protocol that returns results in 73 minutes on QIAGEN's Rotor-Gene Q. The protocol has also been validated for use on other thermal block cyclers. The results are displayed as easy-to-interpret PCR curves.
Applications
The mericon Vibrio triple Kit is for real-time PCR-based detection of Vibrio vulnificus, Vibrio parahaemolyticus, and Vibrio cholerae in food, animal feed, and pharmaceutical products.
Le site web n’est pas disponible en français dans son intégralité. Son contenu est international et peut aller à l’encontre des règlementations locales.
Le site web n’est pas disponible en français dans son intégralité. Son contenu est international et peut aller à l’encontre des règlementations locales.
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