The PyroMark Q96 ID uses proven Pyrosequencing technology for real-time, sequence-based detection and quantification for genetic analysis and epigenetic methylation studies. PyroMark Q96 ID Accessories and Reagents are high-quality reagents and products that simplify and streamline the Pyrosequencing workflow and enable successful results. PyroMark Q96 ID Accessories and Reagents are designed specifically for use with PyroMark Q96 ID instruments.
For 200 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl2 and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl2, and RNase-Free Water
For 800 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl2 and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl2, and RNase-Free Water
PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (50 reactions; plate format); minimum order of 24 assays per plate
PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; plate format); minimum order of 24 assays per plate
PyroMark Q96 ID Accessories and Reagents are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
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Applications
PyroMark Q96 ID Accessories and Reagents enable Pyrosequencing analysis with the PyroMark Q96 ID instrument for applications such as:
HRM
Methylation analysis
Mutation analysis
SNP analysis
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Le site web n’est pas disponible en français dans son intégralité. Son contenu est international et peut aller à l’encontre des règlementations locales.
Kit for optical temperature verification of Rotor-Gene systems, includes a Rotor-Disc 72 preloaded with thermochromatic liquid crystals, fluorescent inserts, CD with calibration files; requires Rotor-Disc 72 Rotor and Locking Ring or Rotor-Disc 72 Starter Kit
For 100 x 50 µl reactions: QIAGEN OneStep RT-PCR Enzyme Mix (1 x 200 µl), 5x QIAGEN OneStep RT-PCR Buffer (1 x 1 ml), dNTP Mix (1 x 200 µl, 10 mM each), 5x Q-Solution (1 x 2 ml), RNase-Free Water (2 x 1.9 ml)
Bag of 50; suitable for holding diluent and master mix on the instrument worktable; graduated, flat-base design with a tapered internal profile for minimum dead volume; screw cap included
