Integrated automated sample preparation and assay setup, with manual transfer to the detection unit, for all molecular testing needs
Unprecedented flexibility
Workflow optimization and utility
Maximized ease of use and convenience
Regulated assays and laboratory-developed tests
Designed for largest range of applications
QIAGEN's premier sample-to-result workflow solution comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and a broad range of ready-to-use assays on the Rotor-Gene Q real-time PCR cycler. QIAGEN continues to expand the QIAsymphony series with new features, assay menus, and workflows.
Explore the virtual demo to learn more about QIAsymphony RGQ.
QIAsymphony SP, QIAsymphony AS, Rotor-Gene Q 5plex HRM; includes required accessories and consumables, installation, and training; includes 1-year warranty on parts and labor
Accessory for correct positioning of the QIAsymphony SP/AS instruments
The QIAsymphony SP is intended to be used only in combination with QIAsymphony Kits indicated for use with the QIAsymphony SP for the applications described in the kit handbooks. If used in combination with QIAGEN kits indicated for use with the QIAsymphony AS instrument, the QIAsymphony AS is intended for the applications described in the respective QIAGEN kit handbooks. If the QIAsymphony AS instrument is used with kits other than QIAGEN kits, it is the users responsibility to validate the performance of such product combination for any particular application. The Rotor-Gene Q, if used in combination with QIAGEN kits indicated for use with the Rotor-Gene Q instrument, is intended for the applications described in the respective QIAGEN kits handbooks. If the Rotor-Gene Q instrument is used with kits other than QIAGEN kits, it is the users responsibility to validate the performance of such product combination for any particular application.
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QIAsymphony workflows.
There are 2 modes of system operation: integrated or independent.
Highly sensitive detection of CMV DNA.
Probit analysis: CMV (QIAsymphony RGQ system). Analytical sensitivity of the artus CMV QS-RGQ Kit with the QIAsymphony RGQ system using EDTA plasma.
Highly sensitive detection of EBV DNA.
Probit analysis: EBV (QIAsymphony RGQ system). Analytical sensitivity of the artus EBV QS-RGQ Kit with the QIAsymphony RGQ system using EDTA plasma.
Highly sensitive detection of HBV DNA.
Probit analysis: HBV (QIAsymphony RGQ system). Analytical sensitivity of the artus HBV QS-RGQ Kit with the QIAsymphony RGQ system.
Highly sensitive detection of HCV RNA.
Probit analysis: HCV (QIAsymphony RGQ system). Analytical sensitivity of the artus HCV QS-RGQ Kit with the QIAsymphony RGQ system.
Highly sensitive detection of HIV-1 RNA
Probit analysis: HI Virus-1 (QIAsymphony RGQ system). Analytical sensitivity of the artus HI Virus-1 QS-RGQ Kit with the QIAsymphony RGQ system.
Performance
The QIAsymphony RGQ, in combination with artus QS-RGQ Kits, enables highly sensitive and specific detection of:
QIAsymphony RGQ can save you time by processing up to 96 samples, from sample to result, in one integrated run. A dedicated range of kits enable open or closed systems for molecular testing.
QIAsymphony RGQ is the integrated system to maximize your efficiency and optimize your workflows, from sample to result. QIAsymphony RGQ synergizes the automated sample preparation and assay setup on the QIAsymphony SP/AS with manual transfer to the trusted Rotor-Gene Q for detection. The QIAsymphony SP automates sample preparation from a wide range of sample types. The QIAsymphony AS delivers highly reliable and reproducible PCR assay setup and is highly suited for laboratories that perform complex PCR-based molecular testing on a day-to-day basis. The QIAsymphony AS enables setup of multiple assays per run or sample and supports artus QS-RGQ products, for a complete CE-IVD compliant workflow, as well as in-house assays. The Rotor-Gene Q enables sensitive, precise real-time PCR analysis with CE-IVD-marked artus QS-RGQ Kits or in-house assays.
Kits for complete CE-IVD compliant workflow on the QIAsymphony RGQ
Kits
Instrument
Method
QIAsymphony DSP Virus/Pathogen Kits
QIAsymphony SP
Purification of viral nucleic acids or bacterial DNA from a broad range of sample types
artus QS-RGQ Kits
QIAsymphony AS
Real-time PCR (multiplex; sample and internal control)
Kits for in-house tests on the QIAsymphony RGQ
Application
Kits
Instrument
Method
Purification
QIAsymphony DSP AXpH DNA Kit
QIAsymphony SP
Purification of DNA from liquid-based cytology media
QIAsymphony DSP
DNA Kits
QIAsymphony SP
Purification of DNA from human whole blood, buffy coat, tissues, FFPE tissues, cultured cells and bacterial cultures, as well as viral DNA from human whole blood
QIAsymphony DSP Virus/Pathogen Kits
QIAsymphony SP
Purification of viral nucleic acids or bacterial DNA from a broad range of sample types
QIAsymphony Certal Kits
QIAsymphony SP
Purification of residual host cell DNA and viral nucleic acids from bioprocess purification buffer, cell culture supernatant samples, and vaccine preparations
Assay setup — pathogen detection
QuantiFast Pathogen +IC Kits
QIAsymphony AS
Multiplex real-time PCR and RT-PCR using sequence-specific probes
QuantiTect Virus Kits
QIAsymphony AS
Highly sensitive detection of multiple viral DNA and RNA targets in the same tube
QIAGEN OneStep RT-PCR Kit
QIAsymphony AS
Sensitive RT-PCR, with simple setup, using any RNA template
Assay setup — gene expression
Rotor-Gene SYBR® Green Kits
QIAsymphony AS
Ultrafast, precise real-time PCR and RT-PCR using SYBR® Green I
Rotor-Gene Probe Kits
QIAsymphony AS
Ultrafast, precise real-time PCR and RT-PCR using sequence-specific probes
Rotor-Gene Multiplex Kits
QIAsymphony AS
Ultrafast, precise multiplex real-time PCR and RT-PCR using sequence-specific probes
Assay setup — residual DNA detection
Certal Detection Kits
QIAsymphony AS
Duplex real-time PCR detection of resDNA target and a generic internal control
Procedure
The QIAsymphony RGQ workflow starts with purification of nucleic acids on the QIAsymphony SP instrument. The eluates, containing purified nucleic acids from the sample preparation procedure, are transferred to the QIAsymphony AS module, which performs assay setup. The assays are then manually transferred to the Rotor-Gene Q for PCR and data analysis.
To suit individual workflow requirements, there are 2 modes of system operation: integrated or independent. In the integrated mode, samples processed on the QIAsymphony SP are transferred automatically to the QIAsymphony AS, reducing manual handling steps and documentation. For added flexibility, the QIAsymphony SP and AS can also be operated independently of each other in independent mode. See "QIAsymphony workflows".
Applications
The QIAsymphony RGQ is the most versatile system for all molecular testing needs — today and tomorrow. The QIAsymphony RGQ allows consolidation of commerical assays, as well as in-house–developed tests, on a single platform.
The broad range of applications for open and closed systems includes:
artus transplantation panel (CMV, EBV, HSV, BK virus, and VZV)
QIAsymphony SP/AS — relative humidity of 15–75% (noncondensing); maximum 75% relative humidity for temperatures up to 31°C, decreasing linearly to 50% humidity at 32°C
Rotor-Gene Q — relative humidity of 10–75% (noncondensing)
QIAsymphony SP/AS — QIAsymphony SP, 128 x 103 x 73 cm; QIAsymphony AS, 59 x 103 x 73 cm; QIAsymphony SP/AS (integrated operation), 185 x 103 x 73 cm
Rotor-Gene Q — width, 37 cm (14.6 in.); height, 28.6 cm (11.3 in.); depth (without cables), 42 cm (16.5 in.); depth (door open), 53.8 cm (21.2 in.)
Kits or Application Packs designed for this instrument
QIAsymphony SP — QIAsymphony DSP Virus/Pathogen Kits and other QIAsymphony Kits; QIAsymphony AS — artus QS-RGQ Kits (not available in all countries), Rotor-Gene Kits and other QIAGEN real-time and end-point PCR kits
Rotor-Gene Q — artus QS-RGQ Kits (not available in all countries), RG SYBR Green PCR Kits; RG SYBR Green RT-PCR Kit; RG Probe PCR Kits; RG Probe RT-PCR Kit; RG Multiplex PCR Kit
Rotor-Gene Q — up to 6 channels spanning UV to infra-red wavelengths; excitation sources: high energy light-emitting diodes; detector: photomultiplier; acquisition time: 4 s; the software allows to create new excitation/detection wavelength combinations
QIAsymphony SP/AS — 100–240 V AC, 50–60 Hz, 1400 VA, mains supply voltage are not to exceed 10% of nominal supply voltages
Rotor-Gene Q — 100–240 V AC, 50–60 Hz, 520 VA (peak); Power consumption 8 VA (standby); Mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages; F5A 250 V fuse
Processing
QIAsymphony SP/AS — automated
Protocols/main application on this instrument
QIAsymphony SP — purification of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials
QIAsymphony AS — PCR setup
Rotor-Gene Q — gene expression analysis, microRNA detection, virus detection, SNP genotyping, SNP genotyping, High Resolution Melt analysis (HRM)
Samples per run (throughput)
QIAsymphony SP — 1–96 samples in batches of 24
Rotor-Gene Q — tubes 0.2 ml; strip tubes 0.1 ml (4 tubes); Rotor-Disc 72; Rotor-Disc 100; up to 100 samples per run using a Rotor-Disc 100
Software
Rotor-Gene Q — Rotor-Gene Q software, supplied on the provided installation CD
Storage temperature
QIAsymphony SP/AS — 5–40°C (41ºF to 104ºF) in manufacturer's package
Rotor-Gene Q — 15 to 30ºC (59ºF to 86ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 1K2 (IEC 60721-3-1)
Rotor-Gene Q — temperature range, 35 to 99ºC; temperature accuracy, ±0.5ºC (type, measured 30 seconds after clock start); temperature resolution, ±0.02ºC (smallest programmable increment); temperature uniformity, ±0.02ºC; ramp rate (peak ramp rates, air), >15ºC/s heating, >20ºC/s cooling
Transportation conditions
QIAsymphony SP/AS — –25 to 70°C (–13ºF to 158ºF) in manufacturer's package
Rotor-Gene Q — –25 to 60ºC (–13ºF to 140ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 2K2 (IEC 60721-3-2)
Typical run time
Rotor-Gene Q — 40 cycles in 45 min with the QIAGEN RG Kits (assay dependent)
Warranty
Rotor-Gene Q — 1 year on instrument; lifetime guarantee on excitation LEDs
Le site web n’est pas disponible en français dans son intégralité. Son contenu est international et peut aller à l’encontre des règlementations locales.
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Le site web n’est pas disponible en français dans son intégralité. Son contenu est international et peut aller à l’encontre des règlementations locales.
