RNeasy 96 BioRobot 8000 Kit

The RNeasy 96 system provides fast and reproducible total RNA purification for high-throughput gene expression profiling. The RNA is suitable for sensitive applications such as quantitative, real-time RT-PCR and microarray analysis (see figure "High-quality RNA for sensitive analysis of a low-copy transcript"). Sample sizes range from 10 to 5 x 10⁵ cells (see figure "RT-PCR of RNA from as few as 100 cells"), and high-quality RNA can be purified from large numbers of samples (see figure "Reproducible yields of high-quality RNA"). Individual variations are low throughout the entire purification process; TaqMan® threshold-cycle values are easily achieved at the end of the process with a coefficient of variation (CV) less than 3% using the QuantiTect Probe RT-PCR Kit (see figure "Repeatability of fully automated RNA purification").

The RNeasy 96 system provides a standardized, fast, reliable method for isolation of high-quality RNA from large numbers of samples (see table). The simple procedure is key to the speed and efficiency of RNeasy 96 technology. Cells can be grown and directly lysed in 96-well cell-culture dishes. After transfer of the lysates to the wells of the RNeasy 96 plate (see figure "RNeasy 96-well plate"), RNA binds to the silica membrane in each well, and contaminants are washed away. Pure RNA is then eluted in RNase-free water into individual collection tubes suitable for long-term storage and is ready to use for any experiment. Since the RNeasy procedure enriches for RNA species >200 nt, RNA yield does not include 5S rRNA, tRNAs, or other low-molecular-weight RNAs.

Specifications of the RNeasy 96 BioRobot 8000 Kit

* Amounts can vary due to developmental stage, growth conditions used, etc.

Specification	Details
Format	RNeasy 96-well plates with 1.2 ml collection microtubes
Sample source	Animal or human cells
Sample size	10–5 x 105 cells
Binding capacity	Up to 100 µg RNA per well
Yield* from 1 x 105 cells	HeLa: 1.6 µg LMH: 1.3 µg COS-7: 3.1 µg Huh7: 2.0 µg Jurkat: 1.4 µg
Elution volume	45–140 µl

Fully automated total RNA isolation using the RNeasy 96 BioRobot 8000 Kit is performed on the BioRobot 8000 workstation or BioRobot Universal System. The BioRobot worktable has the capacity to purify RNA from up to 192 samples in a single run. With the one-plate protocol, the system can carry out both RNA purification and reaction setup in a single run — from living cell cultures in 96-well plates to RT-PCR mixtures, ready to use in real-time gene expression analysis. Filter tips prevent cross-contamination, and precision robotic handling provides minimal well-to-well variation and high repeatability. RNA purification can also be performed manually on the QIAvac 96 vacuum manifold and/or the 96-Well-Plate Centrifugation System using the RNeasy 96 Kit.

The RNeasy 96 BioRobot 8000 Kit consistently provides the highest-quality RNA for:

• High-throughput RNA applications in gene expression analysis
• Sensitive applications such as quantitative, real-time RT-PCR
• Pharmacological and toxicological research