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EpiTect Control DNA and Control DNA Set

For control experiments during methylation analysis
  • Convenient, ready-to-use, quality-controlled DNA
  • Bisulfite-converted DNA for control experiments
  • Suitable for all methylation analyses
EpiTect Control DNAs are ready-to-use, completely methylated or completely unmethylated bisulfite converted DNAs, and untreated, unmethylated genomic DNA, for standardized and reliable control reactions for methylation analysis. The methylated, bisulfite converted DNA as well as unmethylated, bisulfite converted and unconverted DNA are stored in EB Buffer (10 mM Tris Cl) in a ready-to-use solution.

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产品 货号 目录价：
EpiTect Control DNA, methylated (100)
Methylated and bisulfite converted human control DNA for 100 control PCRs
 59655
询价
EpiTect PCR Control DNA Set (100)
Human control DNA set (containing both bisulfite converted methylated and unmethylated DNA and unconverted unmethylated DNA) for 100 control PCRs
 59695
询价
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EpiTect Control DNA and Control DNA Set适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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Real-time PCR标准品。|HRM定量标准品。|EpiTect Control DNA Pyrogram。|在PCR和甲基化分析中使用EpiTect Control DNA|对WBA DNA进行EpiTYPER MALDI-TOF分析。|表观遗传学研究的标准工作流程。|
将EpiTect Methylated Control DNA和EpiTect Unmethylated Control DNA混合，得到100%、90%、50%、10%和0%的甲基化DNA。使用EpiTect MethyLight Assays对人PITX2基因进行三次重复分析，每个DNA样本取10 ng。结果表明，EpiTect Control DNA可用作未知DNA样本定量的甲基化标准品。|扩增APC基因（结肠腺瘤息肉）启动子区域的CpG岛，使用EpiTect HRM PCR Kit在Rotor-Gene Q实时荧光定量PCR分析仪上通过HRM甲基化分析确定甲基化程度。EpiTect Control DNA Set中甲基化和未甲基化DNA按不同比例混合，作为分析模板。|对MGMT启动子的16个CpG进行完全甲基化（甲基化比率>95%）或完全未甲基化（平均甲基化率0.3%）检测。在PyroMark Q96 ID仪器上对MGMT的PCR产物进行焦磷酸测序，MGMT是参与DNA修复和抗药性的p53相关基因。（数据由Uwe Gerstenmaier, Varionostic GmbH, Ulm提供）。|甲基化特异性引物（M-Primer）仅与完全甲基化且亚硫酸氢盐转化的DNA对照结合。非甲基化DNA的特异性引物（U-Primer）仅与未甲基化的亚硫酸氢盐转化的DNA对照结合。M-Primer或U-Primer都不会与未甲基化的基因组DNA结合。|对未甲基化DNA（UMDNA 1）、甲基化DNA（VIAL A 1）和二者混合物（HTXA 1），在使用EpiTect Whole Bisulfitome Kit扩增前后（WBAUMDNA1, WBAHTXA 1, WBAVIAL A 1）进行MP6位点的甲基化检测。扩增前的甲基化模式与扩增后的DNA非常类似，说明是有扩增的位点全面。（数据由美国Hany Ezzeldin, Mayo Clinic, Rochester提供）。||
性能
EpiTect Control DNAs are highly suited for use as quantification standards for probe-based real-time PCR methylation analysis (see figure "Real-time PCR standards") and high-resolution melting (HRM) methylation analysis (see figure "HRM quantification standards").
原理
The EpiTect Control DNA Set contains all control DNAs needed for standardized and reliable methylation control reactions in a ready-to-use kit format . In methylation-specific PCR, for example, control reactions must be carried out to ensure that PCR probes and primers bind specifically to methylated or unmethylated DNA (see figure Use of EpiTect Control DNA in PCR for methylation analysis). These reactions require various concentrations of completely bisulfite converted control DNA that is fully methylated or fully unmethylated (see figures EpiTect Control DNA Pyrogram and "EpiTYPER MALDI-TOF analysis of WBA DNA"). Additionally, mixtures of these control DNAs can serve as quantification standards when determining degree of methylation in HRM and probe-based methylation experiments.
操作流程
Standardized workflows in epigenetics

Accessing epigenetic information is of prime importance for many areas of biological and medical research — particularly oncology, but also stem cell research and developmental biology. However, the analysis of changes in DNA methylation is challenging, due to the lack of standardized methods for providing reproducible data particularly from limited sample material. With its newly introduced EpiTect solutions, QIAGEN makes available standardized, pre-analytical and analytical solutions from DNA sample collection, stabilization and purification, to bisulfite conversion and real-time or end-point PCR methylation analysis or sequencing (see figure "Standardized workflows in epigenetics").

应用

EpiTect Control DNAs are suitable for use in control reactions for all types of methylation analyses, including:

Evaluating primer specificity for MSP
Quantification standard for HRM and MethyLight PCR
Evaluating primer and probe specificity for MethyLight PCR
Checking efficiency of bisulfite conversion reactions
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特点
参数
应用 End point Methylation Specific PCR (MSP), real-time methylation specific PCR
浓度 10 ng/µl (1 µg in total) excepted the unmethylated control DNA: 50 ng/µl (10 µg in total)
反应次数 for 1000 control PCRs

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安全数据表
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Safety Data Sheets
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图片
Standards for quantitative, real-time PCR analysis
Real-time PCR标准品。
将EpiTect Methylated Control DNA和EpiTect Unmethylated Control DNA混合，得到100%、90%、50%、10%和0%的甲基化DNA。使用EpiTect MethyLight Assays对人PITX2基因进行三次重复分析，每个DNA样本取10 ng。结果表明，EpiTect Control DNA可用作未知DNA样本定量的甲基化标准品。
Standard curve for the quantification of the methylation degree in HRM analysis
HRM定量标准品。
扩增APC基因（结肠腺瘤息肉）启动子区域的CpG岛，使用EpiTect HRM PCR Kit在Rotor-Gene Q实时荧光定量PCR分析仪上通过HRM甲基化分析确定甲基化程度。EpiTect Control DNA Set中甲基化和未甲基化DNA按不同比例混合，作为分析模板。
Typical Pyrograms Obtained for Unmethylated and Methylated EpiTect
EpiTect Control DNA Pyrogram。
对MGMT启动子的16个CpG进行完全甲基化（甲基化比率>95%）或完全未甲基化（平均甲基化率0.3%）检测。在PyroMark Q96 ID仪器上对MGMT的PCR产物进行焦磷酸测序，MGMT是参与DNA修复和抗药性的p53相关基因。（数据由Uwe Gerstenmaier, Varionostic GmbH, Ulm提供）。
Use of EpiTect Control DNA
在PCR和甲基化分析中使用EpiTect Control DNA
甲基化特异性引物（M-Primer）仅与完全甲基化且亚硫酸氢盐转化的DNA对照结合。非甲基化DNA的特异性引物（U-Primer）仅与未甲基化的亚硫酸氢盐转化的DNA对照结合。M-Primer或U-Primer都不会与未甲基化的基因组DNA结合。
EpiTYPER® MALDI-TOF Analysis of Bisulfite Converted and Whole Bisulfitome Amplified (WBA)
对WBA DNA进行EpiTYPER MALDI-TOF分析。
对未甲基化DNA（UMDNA 1）、甲基化DNA（VIAL A 1）和二者混合物（HTXA 1），在使用EpiTect Whole Bisulfitome Kit扩增前后（WBAUMDNA1, WBAHTXA 1, WBAVIAL A 1）进行MP6位点的甲基化检测。扩增前的甲基化模式与扩增后的DNA非常类似，说明是有扩增的位点全面。（数据由美国Hany Ezzeldin, Mayo Clinic, Rochester提供）。
Standardized Workflows in Epigenetics
表观遗传学研究的标准工作流程。