QIAsymphony SP – your flexible solution for nucleic acid extraction

QIAsymphony SP AS
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Why choose QIAsymphony SP for nucleic acid extraction?


QIAsymphony SP provides fast, flexible high-quality nucleic acid purification for a wide range of downstream applications.

Explore the links below to see how others have used QIAsymphony SP in their work.
Diagnostic testing applications
Viral load testing
Post-infection vaccine strategies
Infectious disease serotyping
Antimicrobial resistance research
References
Diagnostic testing applications
Nucleic acid extraction is an integral component of many infectious disease diagnostics methods.

In an investigation comparing group A streptococcus diagnostic methods, Gazzano et al. used QIAsymphony SP to extract genomic DNA for subsequent PCR analyses (1).

Kriegsmann et al. used QIAsymphony SP-extracted DNA from FFPE tissue samples to evaluate mass spectrometry (MS) as a method for HPV detection (2).

Viral load testing
Accurate viral load testing is critical for monitoring chronic viral infection. Disease management, therapeutic decisions and treatment response all depend on reliable results.

Tang et al. examined the interchangeability of quantitative standards for clinical viral load testing (3). Using QIAsymphony SP to extract Epstein-Barr virus (EBV) DNA from patient samples, calibrators and controls, the resulting DNA was suitable for quantitative testing by real-time and digital PCR.

Post-infection vaccine strategies
Chronic infection treatment is a growing area of study. One longstanding goal has been to create post-infection vaccine strategies that improve immune responses in the infected host – ultimately cleansing the individual’s system from infection.

Eberhardt et al. used QIAsymphony SP to extract Rhesus cytomegalovirus (RhCMV) DNA from plasma and oral swab samples for subsequent quantitative real-time PCR (4). Results showed some improvement of viral containment in response to vaccine administration that increased antibody titers to interleukin-10.

Infectious disease serotyping
Serotyping provides valuable information about pathogenic species and subspecies, and the illnesses they cause. Organism identification, outbreak detection and surveillance all rely heavily on serotyping.

Today, sequencing is playing a larger role in serotyping work, and high-quality purified nucleic acids are vital. QIAsymphony SP provides just the high-quality purified nucleic acids necessary for trusted serotyping results.

In a study by Gentle et al., QIAsymphony SP was used to extract DNA from Shigella flexneri for downstream whole-genome sequencing to analyze mismatches between genotypic and phenotypic serotypes (5). Discrepant results between genotypes and phenotypes were found to be due to insertions, deletions or point mutations in the synthesis of O-antigen.

Antimicrobial resistance research
Antimicrobial resistance is an ongoing global challenge. High-quality nucleic acid purification is crucial for understanding and combating emerging superbugs.

QIAsymphony SP was key in a pilot study of azithromycin resistance in Salmonella serovars. Nair et al. used QIAsymphony SP-purified DNA from more than 600 Salmonella isolates for whole genome sequencing (WGS) (6). The study identified, in four different Salmonella serovars, the presence of macrolide resistance genes mphA, mphB and mefB – each of which were associated with increased azithromycin resistance.

Learn more about QIAsymphony SP now.

References
  1. Gazzano, V. et al. (2016) Reassessment of the role of rapid antigen detection tests in diagnosis of invasive group A streptococcal infections. J Clin Microbiol 54(4), 994–999. Link
  2. Kriegsmann, M. et al. (2017) Detection of HPV subtypes by mass spectrometry in FFPE tissue specimens: a reliable tool for routine diagnostics, J Clin Pathol 70(5), 417–423.
  3. Tang, L. et al. (2016) Quantitative assessment of commutability for clinical viral load testing using a digital PCR-based reference standard. J Clin Microbiol 54(6), 1616–1623. Link
  4. Eberhardt M.K., et al. (2016) Exploitation of interleukin-10 (IL-10) signaling pathways: alternate roles of viral and cellular IL-10 in Rhesus cytomegalovirus infection. J Virol. 90(21), 9920–9930. Link
  5. Gentle, A., Ashton, P.M., Dallman, T.J., and Jenkins, C. (2016) Evaluation of molecular methods for serotyping Shigella flexneri. J Clin Microbiol 54(6), 1456–1461. Link
  6. Nair, S. et al. (2016) WGS for surveillance of antimicrobial resistance: a pilot study to detect the prevalence and mechanism of resistance to azithromycin in a UK population of non-typhoidal Salmonella. J Antimicrob Chemoth 71(12), 3400–3408. Link

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