Highly specific cloning with a shorter ligation time of 30 min.
Highly specific cloning with a shorter ligation time of 30 min.
Highly specific cloning with a ligation time of 4 h.
Highly specific cloning with a ligation time of 4 h.
The QIAGEN PCR Cloning Kit procedure.
The QIAGEN PCR Cloning Kit procedure.
pDrive Cloning Vector.
pDrive Cloning Vector.
Highly specific cloning with a shorter ligation time of 30 min. The effect of ligation time on cloning efficiency was compared for the QIAGEN PCR Cloningplus Kit and a TA-based cloning kit (Supplier I) using PCR products of different length (0.5 kb, 1 kb, and 3 kb). The recommended protocol for each kit was followed. Colony numbers were converted to relative percentages, with the QIAGEN PCR Cloningplus Kit procedure set at 100% for each comparison. The ligation was performed for 30 min (QIAGEN PCR Cloningplus Kit recommendation).
Highly specific cloning with a ligation time of 4 h. The effect of ligation time on cloning efficiency was compared for the QIAGEN PCR Cloningplus Kit and a TA-based cloning kit (Supplier I) using PCR products of different length (0.5 kb, 1 kb, and 3 kb). The recommended protocol for each kit was followed. Colony numbers were converted to relative percentages, with the QIAGEN PCR Cloningplus Kit procedure set at 100% for each comparison. The ligation was performed for 4 hours (TA-based cloning recommendation).
The QIAGEN PCR Cloning Kit procedure.

Simply mix the PCR product directly with pDrive Cloning Vector and Ligation Master Mix, incubate, and then add the ligation reaction to competent cells for transformation.
pDrive Cloning Vector.

The pDrive Cloning Vector has a number of useful features designed to facilitate analysis of cloned PCR products. These include a large number of unique restriction enzyme recognition sites, universal sequencing primer sites, and promoters for in vitro transcription. In addition, the vector allows both ampicillin and kanamycin selection, as well as blue/white screening of recombinant colonies.