Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp 96-well plate. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer.
Gel electrophoresis of 96 DNA preparations purified from a single sample of whole human blood using the QIAamp 96 DNA Blood Kit. End lanes contain lambda-HindIII markers.
[A] DNA yields (in µg) and [B] purity of 96 preparations isolated from a single donor and purified using the QIAamp 96 DNA Blood Kit. Mean values and standard errors from ten such experiments are shown.