Figure 1: Isolate more high-quality genomic DNA.
Figure 1: Isolate more high-quality genomic DNA.
Figure 2: Improved, high-quality DNA yields with the new DNeasy PowerSoil Pro Kit.
Figure 2: Improved, high-quality DNA yields with the new DNeasy PowerSoil Pro Kit.
Figure 3: Increased bacterial OTUs with the new DNeasy PowerSoil Pro Kit.
Figure 3: Increased bacterial OTUs with the new DNeasy PowerSoil Pro Kit.
Figure 4: Increased purity and less variability in DNA extracted with the DNeasy PowerSoil Pro Kit.
Figure 4: Increased purity and less variability in DNA extracted with the DNeasy PowerSoil Pro Kit.
Figure 1: Isolate more high-quality genomic DNA. 250 mg of the three different soil types were prepared using commercially available sample preparation solutions and compared to the new DNeasy PowerSoil Pro Kit. The DNeasy PowerSoil Pro Kit out-performed other available kits, resulting in 8-fold greater recovery of DNA across the variety of soil types.
Figure 2: Improved, high-quality DNA yields with the new DNeasy PowerSoil Pro Kit. Gel A shows 1.8 ml of Candida albicans fungal culture processed with the DNeasy PowerSoil Kit (a) compared with the DNeasy PowerSoil Pro Kit (b). Gels B and C contain various soil samples (250 mg). Gel B was processed with the original DNeasy PowerSoil Kit, while Gel C was processed using the DNeasy PowerSoil Pro Kit. As evidenced by the stronger gel bands, a greater DNA yield was isolated from the sample using the DNeasy PowerSoil Pro Kit.  
Figure 3: Increased bacterial OTUs with the new DNeasy PowerSoil Pro Kit. DNA prepared from soil samples was isolated with different methods and enriched. Analysis of the 16S rRNA-genes was done using the QIAseq 1-Step Amplicon Kit and data analysis was done using the Microbial Genomics Pro suite (CLC workbench). Alpha diversity was determined by total number of bacterial operational taxonomic units (OTUs). The greatest diversity was seen in the samples processed with the DNeasy PowerSoil Pro Kit.
Figure 4: Increased purity and less variability in DNA extracted with the DNeasy PowerSoil Pro Kit. Soil samples (250 mg each) were prepared using commercially available sample preparation solutions and compared to the new DNeasy PowerSoil Pro Kit. DNA purity was depicted via UV measurements and the analysis of ratios A260/A280, A, and A260/A230, B. Inhibitor removal was visualized with an inhibitor-sensitive PCR with an internal control spiked with eluates from the PureLink Microbiome DNA Purification Kit and the DNeasy PowerSoil Pro Kit. Eluate-free reactions served as internal control, C. DNA extracted with the DNeasy PowerSoil Pro Kit shows an increased purity compared with other sample preparation kits with no inhibition of PCR.