The dPCR LNA Mutation Assays are duplex, hydrolysis probe-based assays that identify the presence of specific mutation sequences. One probe detects the mutant allele, and the other probe detects the wild-type allele. Digital PCR provides the most sensitive and reliable method for detecting a mutant DNA sequence in a wild-type background. dPCR LNA Mutation Assays use LNA-enhanced primers and probes, which increase assay specificity and sensitivity, making it possible to detect a mutation present at 0.1% in a background of wild-type genomic DNA in a single nanoplate well.
Two fluorescence detection options enable multiplex analysis of two mutation targets in one well. During product configuration, the choice of FAM + HEX or Atto 550 + ROX for detecting mutant + wild-type is available.