An independent study shows that Ni-NTA loses less nickel than any other tested resin.
An independent study shows that Ni-NTA loses less nickel than any other tested resin. Fifty column volumes of buffer containing various additives was passed through a small column containing 100 μl resin from QIAGEN, Supplier G, S, or I. The flowthrough was pooled and a sample sent for analysis by inductively coupled plasma mass spectrometry (ICP-MS) at Dr.Weßling Laboratories, Bochum, Germany according to DIN EN ISO 17025. Native buffer: 50 mM Na phosphate; 300 mM NaCl; 10 mM imidazole, pH 8.0. Denaturing buffer: 100 mM Na phosphate; 10 mM Tris·Cl; 8 M urea.