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TaqNova Master Mix 2x (100 U)
Cat. No. / ID: RP85T
100 rxns
Components: 2 x 1.25 mL of TaqNova Master Mix 2x, 2 x 1.5 mL PCR-grade water. Formulation: 2x concentrated PCR reaction buffer, 4 mM MgCl2; 1.6 mM dNTPs mix (0.4 mM of each dNTP); 0.04 U/μL TaqNova DNA Polymerase, red inner dye and density reagent. Store all components at -20°C. After thawing, the product will remain stable at 4°C for at least two months. Up to 10 freeze cycles will not compromise product performance.
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Reactions
100 U
1000 U
The TaqNova Master Mix is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Need bulk, customized or optimized products for commercial purposes? We also offer support with logistics, compliance and more. Reach out to cooperate with QIAGEN Strategic Partnerships & OEM
Features
- Recombinant enzyme of high purity with a half-life of 45 minutes at 95°C
- Amplifies fragments of up to 5 kb
- Leaves ´A´ overhangs
- Facilitates high throughput PCR reaction setup with a reduced risk of contamination
Product Details
2x PCR TaqNova-RED is a 2x concentrated, ready-to-use PCR master mix that facilitates an easy and rapid PCR reaction setup. The 2x PCR TaqNova-RED solution contains a reaction buffer, magnesium chloride, dNTPs and a thermostable Taq DNA polymerase.
It is supplied with 20 mM Tris-HCl (pH 8.0 at 25°C), 200 mM KCl, 0.1 mM EDTA, 2 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol.
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C in a 50 μL reaction.
Performance
| Assay | Specification |
|---|---|
| Functional activity | Passed |
| Unspecific nuclease contamination | None detected |
Principle
To set up the PCR reaction, all that needs to be added to the master mix is the template, the primer set and water. This convenient 2x PCR TaqNova-RED master mix reduces the time required to set up PCR reactions and decreases the possibility of contamination, particularly when preparing large volumes of reactions. Moreover, the master mix includes an inner dye and a density reagent, which allows the direct loading of PCR products into a gel.
Procedure
Quality Control
The master mix is free of unspecific nucleases contamination and is extensively tested for PCR applications.
Applications
This is used for standard and specialized applications such as:
- Amplification of short and medium size DNA sequences
- Routine and diagnostic PCR
- TA cloning
- Multiplex PCR assays
Resources
Quick-Start Protocols (1)
Safety Data Sheets (1)
Certificates of Analysis (1)