QIAamp DSP Virus Kit

Pour la purification d’acides nucléiques viraux à partir de plasma humain et de sérum dans le cadre du diagnostic in vitro

S_1084_5_GEN_disclaimer

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

QIAamp DSP Virus Kit

Cat. No. / ID:  60704

For 50 preps: QIAamp MinElute Columns, buffers, reagents, tubes, column extenders, VacConnectors
Copy order details
447,00 €
Log in To see your account pricing.
1. Manuel du kit QIAamp DSP Virus © QIAGEN France S.A.S. 2013 Les informations relatives aux dispositifs médicaux in vivo et in vitro contenues dans ce site sont à destination des professionnels de santé. Pour plus d'informations, nous vous invitons à consulter le manuel d'utilisation de l'équipement et/ou la notice d'utilisation du réactif.
Le QIAamp DSP Virus Kit est destiné à un usage de diagnostic in vitro.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Système universel de purification compatible avec d’autres produits CE-IVD
  • Acides nucléiques viraux de haute qualité (1)
  • Concentration avec un volume d'élution de 20 µl ou 60 µl (1)
  • Volumes d’échantillon de 500 µl (1)
  • Purification rapide et risque minime de contamination croisée (1)

Product Details


Le kit QIAamp DSP Virus se base sur la technologie QIAamp pour la purification simultanée de l’ADN et de l’ARN viraux dans le cadre du diagnostic in vitro.

Performance

Les acides nucléiques viraux purifiés à l’aide du kit QIAamp DSP Virus sont prêts à être utilisés dans le cadre d’applications sensibles en aval, telles que celles fondées sur l’amplification enzymatique ou une autre modification notamment la PCR et la RT-PCR.

Principle

Le kit QIAamp DSP Virus se base sur la technologie QIAamp pour la purification simultanée d'ADN et dl’ARN viral. La membrane de silice de QIAamp lie les acides nucléiques dans l’échantillon lysé, tandis que le reste du lysat est rapidement retiré par aspiration sous vide. Les acides nucléiques liés sont lavés de manière efficace afin d’éliminer les contaminants puis élués dans un volume de 20 à 60 µl.

Procedure

La procédure du kit QIAamp DSP Virus inclut les étapes suivantes : lyse, liaison, lavage (3x), séchage par centrifugation et élution. Les acides nucléiques viraux sont purifiés à partir de plasma ou de sérum à l’aide d'une chambre à vide vide, tel que le système QIAvac 24 Plus (voir le schéma « Procédure »).

Applications

Les acides nucléiques viraux peuvent être purifiés à partir d’échantillons de plasma ou de sérum. Les échantillons peuvent contenir des anticoagulants (EDTA ou citrate) et peuvent être frais, lyophilisés ou congelés (à condition qu’ils n’aient pas été décongelés puis recongelés).

Le kit QIAamp DSP Virus permet la purification des acides nucléiques à partir d’une grande variété de virus. Ce kit est compatible avec un vaste panel de systèmes de prélèvement d’échantillons en amont et d’applications en aval, il peut donc facilement s’intégrer au déroulement des opérations du diagnostic.

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsDownstream detection procedures in molecular diagnostics, such as PCR
Elution volume20 µl, 60 µl
Main sample typeSerum, plasma
CE/FDA/IVD compatibleCE/IVD
FormatSample tubes
ProcessingManual (vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinViral DNA, viral RNA
Sample amount500 µl
TechnologySilica technology
YieldVaries

Resources

Kit Handbooks (1)
For the EU IVDR 2017/746 compliant kit (Kit Version 2)

June 2022
Safety Data Sheets (1)
Brochures & Guides (1)
Performance Data (1)
For use with QIAamp DSP Virus Kit

June 2022
Certificates of Analysis (1)

Publications

Occurrence of hepatitis A virus genotype III in Germany requires the adaptation of commercially available diagnostic test systems.
Heitmann A; Laue T; Schottstedt V; Dotzauer A; Pichl L;
Transfusion; 2005; 45 (7):1097-105 2005 Jul PMID:15987353

FAQ

Does the QIAamp DSP Virus Kit require the QIAvac 24 Plus, or can I use it with my own laboratory vacuum system?
It is possible to use a general laboratory vacuum system with the QIAamp DSP Virus Kit. However, this will require validation in-house. The CE-marked QIAamp Kits have been developed, field-tested and validated with the QIAvac 24 Plus vacuum system (QIAvac 24 Plus, QIAvac Connecting System and Vacuum Pump). Using this system with the QIAamp DSP Virus Kit will avoid lengthy validation procedures for the enduser.
FAQ ID -789
What vacuum pressure should be applied using the QIAvac 24 Plus for the QIAamp DSP Virus Kit and Blood Mini Kit Protocols?

When processing samples with the QIAamp DSP DNA Blood Mini Kit and the QIAamp DSP Virus Kit, the vacuum pressure should be below -800 mbar. The QIAvac 24 Plus and the QIAvac Connecting System should be tested according to Appendix B of the QIAvac 24 Plus Handbook before performing a nucleic acid purification procedure.

If you would like to use an equivalent general laboratory vacuum system, make sure that the minimum vacuum pressure is reached. Performance of the QIAamp DSP DNA Blood Mini or Virus Kits in combination with a house-vacuum system has to be validated by the researcher.

FAQ ID -1033
What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

FAQ ID -761
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728
What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?

For RNA isolated on the BioRobot EZ1 and BioRobot M48:

The RNA can be directly applied to the Agilent Bioanalyzer, since it is being denatured in the final protocol steps of these isolation procedures.

For RNA prepared with all other QIAGEN RNA Isolation Products:

We recommend to denature the samples in a water bath for 2 min at 70°C, and then place them directly on ice prior to loading them onto the Agilent Bioanalyzer.

 

FAQ ID -528
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12