Can miRNA sequencing libraries be prepared from total RNA isolated from heparinized plasma samples?

Heparin is a potent reverse-transcription inhibitor. After total RNA isolation from heparinized plasma samples, the heparin must be removed. One option is to treat the total RNA samples with heparinase prior to library construction with the QIAseq miRNA Library Kit. 

icon_0011_idea-s

Can’t find what you are looking for?

Browse the FAQ base with our FAQ search.