RT2 lncRNA PCR Array Modification
For lncRNA expression profiling using a partially modified pathway or disease panel
The RT2 lncRNA PCR Array Modification combines the same expertly selected lncRNA panels present in cataloged RT2 lncRNA PCR Arrays with up to 4 lncRNAs unique to your research. Add any 4 lncRNA assays from a cataloged array and replace them with lncRNA assays of your choice, selected from our comprehensive list of human or mouse RT2 lncRNA qPCR Assays.
To order a RT2 lncRNA PCR Array Modification, please use this excel file.
RT2 lncRNA PCR Arrays are panels of RT2 lncRNA qPCR Assays for long noncoding RNAs related by pathway or disease. Assays are designed using a computer algorithm, which has been developed using an in vitro assay to ensure that the resulting primer sequences generate a single PCR product of the predicted size without primer-dimers or off-target amplification – in 33 cycles of PCR amplification. The assay also ensures that the amplification efficiency of the primers is 100 +/– 10%. As a result, the algorithm designs highly effective primer sequences for SYBR® Green based real-time PCR detection.
For optimal performance, RT2 lncRNA PCR Arrays should be used together with the RT2 First Strand Kit for cDNA synthesis and RT2 SYBR Green Mastermixes for qPCR. These reagents have been formulated and pretested together with RT2 lncRNA PCR Arrays. The RT2 First Strand Kit includes a proprietary genomic DNA elimination step to remove any residual contamination in RNA samples before reverse transcription, thereby eliminating false positive signals. In addition, an artificial RNA control template is also included which can be used to follow the reverse transcription efficiency across samples and monitor against RNase contamination. Each of the real-time instrument-specific RT2 SYBR Green Mastermixes contains SYBR Green and an appropriate reference dye to match the instrumentation available in your laboratory. RT2 SYBR Green Mastermixes are available for all real-time PCR instruments from QIAGEN, Applied Biosystems, Bio-Rad, Stratagene, Eppendorf, Roche and other major suppliers.
Simply mix the cDNA template with the appropriate ready-to-use PCR mastermix, aliquot equal volumes to each well of the same plate and run the real-time PCR cycling program (see flowchart "Simple procedure"). RT2 lncRNA PCR Arrays are compatible with all QIAGEN, ABI, Bio-Rad, Eppendorf, Roche and Stratagene instruments.
RT2 lncRNA PCR Array Modification is highly suited for lncRNA research that requires the study of a few specific lncRNAs in combination with overall pathway or disease profiling.
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