miScript miRNA Mimics

For studies on miRNA function and gene regulation using synthetic miRNA

  • Ready-to-transfect mimics behave like endogenous miRNAs
  • Available for all human, mouse, and rat miRNAs in miRBase
  • Highly flexible formats, scales, and grades
  • Custom design available for miRNAs not listed in miRBase
  • Easy to order via GeneGlobe

miScript miRNA Mimics are chemically synthesized, double-stranded RNAs which mimic mature endogenous miRNAs after transfection into cells. miScript miRNA Mimics are available at cell-culture grade (>90% purity) or animal grade (HPLC purified; for in vivo applications). For flexible screening, miScript miRNA Mimic Plates enable researchers to order any predesigned or custom-designed miScript miRNA Mimics in their choice of layout in 96-well or 384-well plates. miScript miRNA Inhibitors are suitable for similar experiments. For transfection, QIAGEN provides HiPerFect Transfection Reagent for miRNA Mimic transfection and Attractene Transfection Reagent for miRNA Mimic-DNA cotransfection.

 

Please beware that miScript miRNA Mimics will be discontinued on July 1, 2019. Products will still be available for reordering, but the product webpage will be removed. We strongly recommend miRCURY LNA miRNA Mimics which have superior performance.

 

Search in GeneGlobe
You can search for the following terms:
  • Entrez Gene IDs (e.g., 835)
  • RefSeq IDs (e.g., NM_032983, NP_116765)
  • Gene symbols (e.g., CASP2)
  • Cat. no. (e.g., SI00299551, QT01342509)
  • Sanger ID or Accession (e.g., hsa-let-7b, MI0000063)
  • CpG loci identification numbers (CG#) (e.g., CG17753661)

Do not enter species information in the Search box. Use the drop-down list to select your species of interest.
When searching for miRNAs, do not omit the hyphens. Use hyphens or spaces (e.g., search for hsa-let-7b or hsa let 7b. Do not search for hsalet7b).

Advanced search settings



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To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
产品 货号 目录价:
miScript miRNA Mimic
1 nmol (cell-culture grade), 5 nmol (cell-culture grade), or 20 nmol (animal grade) miScript miRNA Mimic, provided in tubes
Varies
Details

Can’t order online or need plate formats? Order Forms and Plate Orders

miScript miRNA Mimics适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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内源性miRNA和miScript miRNA Mimic的下调效果相当。|miScript miRNA Mimic可保持稳定长达72小时。|miR-1模拟物转染后在蛋白水平的下调作用。|
miR-1在HeLa S3细胞中呈非内源性表达。miR-16在HeLa S3细胞中呈高水平的内源性表达(Lagos-Quintana, M. et al. [2001] Identification of novel genes coding for small expressed RNAs. Science 294, 853.)。使用HiPerFect Transfection Reagent,将3' UTR带有miR-1结合位点的荧光素酶报告基因重组体转染至HeLa S3细胞(6 x 104个细胞/孔);共转染3' UTR带有miR-1结合位点的荧光素酶报告基因重组体及miR-1 Mimic;或者转染3' UTR带有miR-16结合位点的荧光素酶报告基因重组体。24小时后,检测荧光素酶活性。miR-1 Mimic对荧光素酶水平的下调效果类似内源性miR-16。|使用HiPerFect Transfection Reagent,将阴性对照或miR-1 Mimic转染至HeLa S3细胞(6 x 104个细胞/孔)。24小时后检测,并使用Attractene Transfection Reagent,将带有miR-1结合位点的荧光素酶报告基因重组体转染至细胞内。在标示的时间点后,通过检测荧光素酶活性检测miR-1水平。|HDAC4预计为miR-1的靶点,且在3' UTR有2个miR-1结合位点(Chen, J.F. et al. [2006] The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation. Nat. Genet. 38, 228.)。使用HiPerFect Transfection Reagent,将各种浓度的miR-1 Mimic转染至HeLa S3细胞(8 x 104个细胞/孔)。miR-1 #1、miR-1 #2和miR-1 #3是不同批次的miR-1 Mimic。24和48小时后,[A] 利用western印迹分析HDAC4蛋白水平。同时分析微管蛋白,作为内部对照(未显示)。24小时后未观察到任何反应。48小时后,蛋白水平显著降低。[B] 使用50 nM miR-1 Mimic转染后,使用miScript System,利用real-time PCR分析HDAC4转录本的水平。阴性对照为AllStars Negative Control。采用GAPDH和阴性对照对结果进行标准化,以2-ΔΔCT表示。在24小时或48小时,转录本水平未发生明显改变。|
性能
miScript miRNA Mimics cause a decrease in gene expression similar to that observed with endogenous miRNAs (see figure "Comparable downregulation by endogenous miRNA and miScript miRNA Mimic"). The observed decrease in gene expression is often mediated at the translational level (see figure "Downregulation at protein level after miR-1 Mimic transfection"). miScript miRNA Mimics efficiently inhibit gene expression for up to 72 hours after transfection (see figure "miScript miRNA Mimic stable for 72 hours").
原理

Transfection of mimics followed by downstream gene expression analysis or phenotypic analysis, is performed to elucidate the targets and roles of particular miRNAs. These experiments enable study of the biological effects of misregulation of individual miRNAs as well as confirmation of specific genes as targets of individual miRNAs. Reduced gene expression after transfection of mimic provides evidence that the miRNA under study is involved in regulation of that gene. miScript miRNA Inhibitors can also be used for similar studies. Typically, such experiments involve transfection of mimic or inhibitor, or alternatively cotransfection with a vector construct which carries the miRNA-binding site fused to a reporter gene. Downstream analysis can include reporter assays, real-time PCR, microarray analysis, or protein analysis.

操作流程

miScript miRNA Mimics are available for every human, mouse, rat, and virus miRNA in the current version of miRBase, the microRNA database. QIAGEN's GeneGlobe database is continually updated to ensure that mimic and inhibitor designs match the most up-to-date version of miRBase. This ensures that you are always working with the most accurate and current miRNA sequence information possible. If the miRNA of interest is not available in miRBase, a mimic for your human, mouse, rat, or virus miRNA can be custom synthesized. Simply visit the custom miScript miRNA product design page, enter a name for the mimic, provide the target sequence, and our design tool will provide a custom mimic ready to order.

Flexible formats enable either low-throughput analysis of small numbers of mimics or high-throughput screening of hundreds of mimics. miScript miRNA Mimics are available in tubes (1 nmol or 5 nmol) at cell-culture-grade (>90% purity provided by proprietary synthesis and purification processes). For in vivo applications, HPLC-purified, animal-grade miScript miRNA Mimics can be ordered at the 20 nmol scale (provided in tubes). For medium- to high-throughput applications, 96-well plates (1 nmol or 5 nmol), or 384-well plates (1 nmol) are available to suit your experimental requirements. Simply upload an Excel sheet of the predesigned (listed in miRBase) or custom (not listed in miRBase) miScript miRNA Mimics in your preferred plate layout, and then view and order the plate.

应用
miRNA research
Gene regulation research
特点
参数
设计 Predesigned and custom design avaliable
规格 Single tubes, 96-well & 384 well plates
修饰 None
规格 1 nmol, 5 nmol, 20 nmol
提供的序列 No
物种 Human, mouse, rat

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图片
Comparable Downregulation by Endogenous miRNA and miScript miRNA Mimic Comparable Downregulation by Endogenous miRNA and miScript miRNA Mimic
内源性miRNA和miScript miRNA Mimic的下调效果相当。
miR-1在HeLa S3细胞中呈非内源性表达。miR-16在HeLa S3细胞中呈高水平的内源性表达(Lagos-Quintana, M. et al. [2001] Identification of novel genes coding for small expressed RNAs. Science 294, 853.)。使用HiPerFect Transfection Reagent,将3' UTR带有miR-1结合位点的荧光素酶报告基因重组体转染至HeLa S3细胞(6 x 104个细胞/孔);共转染3' UTR带有miR-1结合位点的荧光素酶报告基因重组体及miR-1 Mimic;或者转染3' UTR带有miR-16结合位点的荧光素酶报告基因重组体。24小时后,检测荧光素酶活性。miR-1 Mimic对荧光素酶水平的下调效果类似内源性miR-16。
miScript miRNA Mimic Stable for 72 Hours
miScript miRNA Mimic可保持稳定长达72小时。
使用HiPerFect Transfection Reagent,将阴性对照或miR-1 Mimic转染至HeLa S3细胞(6 x 104个细胞/孔)。24小时后检测,并使用Attractene Transfection Reagent,将带有miR-1结合位点的荧光素酶报告基因重组体转染至细胞内。在标示的时间点后,通过检测荧光素酶活性检测miR-1水平。
Downregulation at Protein Level after miR-1 Mimic Transfection
miR-1模拟物转染后在蛋白水平的下调作用。
HDAC4预计为miR-1的靶点,且在3' UTR有2个miR-1结合位点(Chen, J.F. et al. [2006] The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation. Nat. Genet. 38, 228.)。使用HiPerFect Transfection Reagent,将各种浓度的miR-1 Mimic转染至HeLa S3细胞(8 x 104个细胞/孔)。miR-1 #1、miR-1 #2和miR-1 #3是不同批次的miR-1 Mimic。24和48小时后,[A] 利用western印迹分析HDAC4蛋白水平。同时分析微管蛋白,作为内部对照(未显示)。24小时后未观察到任何反应。48小时后,蛋白水平显著降低。[B] 使用50 nM miR-1 Mimic转染后,使用miScript System,利用real-time PCR分析HDAC4转录本的水平。阴性对照为AllStars Negative Control。采用GAPDH和阴性对照对结果进行标准化,以2-ΔΔCT表示。在24小时或48小时,转录本水平未发生明显改变。