Attractene Transfection Reagent

For efficient DNA transfection and DNA–siRNA/miRNA cotransfection
  • Highly efficient DNA transfection with extremely low cytotoxicity
  • Rapid Fast-Forward Protocol
  • Reagent of choice for all adherent cells and sensitive cells
  • Ideal for cotransfection and vector-based RNAi
  • Free of animal-derived components

Attractene Transfection Reagent represents the next generation in lipid technology, ensuring highly efficient DNA transfection of eukaryotic cells. Attractene Reagent is a nonliposomal lipid that enables transfection of all adherent cells, including difficult-to-transfect cell types such as HaCaT, MonoMac6, and HCT116, and some suspension cell types (Jurkat, K562). It is also highly suitable for cotransfection of DNA with siRNA or miRNA mimics or inhibitors.

Please note: The product 1051561: Attractene Transfection Reagent (0.1 ml) is phasing-out by December 31, 2015 and available only while stock lasts. Please contact your customer care department. Please use product 301005: Attractene Transfection Reagent (1 ml) as alternative.

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产品 货号 目录价:
Attractene Transfection Reagent (1 ml)
Attractene Transfection Reagent for up to 660 transfections in 24-well plates
301005
$779.00
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Attractene Transfection Reagent (4 x 1 ml)
Attractene Transfection Reagent for up to 2640 transfections in 24-well plates
301007
$2,556.00
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Attractene Transfection Reagent适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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Attractene Reagent的性能优于其他同类试剂。|使用Attractene Reagent转染后的健康细胞。|灵活且快速的Fast-Forward实验方案。|shRNA载体转染后的高效敲除。|
参照Attractene Reagent操作手册推荐的条件,使用Attractene Reagent将DNA(表达绿色荧光蛋白的pGFP)转染至指定的细胞类型中。同时使用生产商推荐的2倍试剂体积的[A] Reagent F进行转染,或者参照生产商的推荐,在提供及不提供增强剂的条件下使用[B] Reagent L进行转染。通过FACS分析计数荧光细胞数,评估转染效率。将使用Attractene Reagent的效率设置为100%,转染效率以与其的相对值表示。|使用Attractene Transfection Reagent或另一家供应商提供的Reagent L,参照生产商的说明将DNA(pGFP)转染至HepG2细胞。FACS分析证明,在两种培养物中含有相同数量的转染细胞。转染后两天,采用光学显微镜观察细胞。[A] 使用Attractene Reagent转染的细胞健康且有活性。[B] 而使用Reagent L转染的细胞的死亡率较高。[C] 使用CellTiter-Blue Assay (Promega) 检测细胞活性。使用Reagent L转染的细胞的活性明显低于使用Attractene Reagent转染的细胞(设置为100%)。|传统上,在转染前一天接种细胞。使用Fast-Forward实验方案,可在转染当天接种细胞,节省了时间和精力。|[A] 仅将表达绿色荧光蛋白(pGFP)的质粒转染至HEK293细胞,或者将[B] pGFP和表达针对绿色荧光蛋白基因的shRNA的质粒(pGFP + pshGFP)共转染至HEK293细胞,或者将[C] pGFP和表达scrambled shRNA的阴性对照质粒(pGFP + pNegative)共转染至HEK293细胞。在完成pGFP及shRNA载体pshGFP共转染后,绿色荧光蛋白被高效沉默,说明共转染高效完成。|
性能

Attractene Reagent provided higher transfection efficiency than alternative reagents when transfection was performed without any optimization following the recommended conditions provided in the handbooks (see figure "Attractene Reagent outperforms alternative reagents"). Cytotoxicity must be kept to a minimum to ensure that results are due to the nucleic acid transfected and not the transfection process itself. Attractene Reagent ensures exceptionally low cytotoxicity (see figure "Healthy cells after transfection using Attractene Reagent"). Using Attractene Reagent ensures that transfection of shRNA (short-hairpin RNA) vectors for gene silencing experiments can be achieved with high efficiency (see figure "Effective knockdown after shRNA vector transfection").

 

 

原理

Attractene Reagent is a nonliposomal lipid which enables highly efficient DNA transfection of some suspension cell types (Jurkat, K562), and adherent cells, including difficult-to-transfect cell types such as HaCaT, MonoMac6, and HCT116. Attractene Reagent consistently provides higher transfection efficiency than alternative reagents under a range of experimental conditions. This feature allows flexible handling and ease of use for many experimental setups such as using automated platforms and transfection at high- or low-throughput levels. It is also highly suitable for cotransfection of DNA with siRNA or miRNA mimics or inhibitors. Using Attractene Reagent ensures exceptionally low cytotoxicity, which is critical to successful transfection experiments.

操作流程

Attractene Transfection Reagent is highly suited for rapid fast-forward DNA transfection. In Fast-Forward Protocols, cells are seeded and transfected on the same day (see figure "Flexible, rapid Fast-Forward Protocols"). This is quicker, saves labor, and increases experimental flexibility compared to protocols where cells are seeded the day before transfection.

Cell type-specific protocols at the TransFect Protocol Database

In addition to the protocols provided in the Attractene Transfection Reagent Handbook, you can find protocols to suit your cell type and plate/dish format using QIAGEN's TransFect Protocol Database. The database provides exactly the protocol needed, saving time and effort. Simply enter the cell type and nucleic acid of interest to receive a QIAGEN transfection protocol to print out or download in convenient PDF format. Use of the TransFect Protocol Database is free of charge and no registration is required.

 

应用

Attractene Transfection Reagent can be used for a range of transfection applications, including transient or stable transfection, or cotransfection of multiple DNA constructs, and cotransfection of DNA with siRNA or miRNA mimics or inhibitors. Attractene Transfection Reagent can be used in:

Functional genomics
High-throughput DNA transfection
Gene silencing
特点
参数
应用 Plasmid transfection, shRNA vector transfection, protein overexpression, reporter studies, RNAi experiments
细胞类型 All cell types including sensitive and difficult to transfect cells
对照 Not included
特点 Highly efficient transfection with lowest toxicity. Rapid fast forward transfection protocol. Free of animal-derived components. Transfection in the presence of serum.
核酸 DNA
可转染的次数 up to 660 transfections in 24-well plates / 1 ml reagent
技术 Cationic lipid based transfection reagent
转染类型 Transient transfection, stable transfection, plasmid co-transfection

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Brochures & Guides
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Brochure detailing reagents for efficient and robust DNA and RNA transfection.
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Kit Handbooks
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For efficient DNA transfection of a broad range of cell lines, including sensitive cell types
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图片
Attractene Reagent Outperforms Alternative Reagents
Attractene Reagent的性能优于其他同类试剂。
参照Attractene Reagent操作手册推荐的条件,使用Attractene Reagent将DNA(表达绿色荧光蛋白的pGFP)转染至指定的细胞类型中。同时使用生产商推荐的2倍试剂体积的[A] Reagent F进行转染,或者参照生产商的推荐,在提供及不提供增强剂的条件下使用[B] Reagent L进行转染。通过FACS分析计数荧光细胞数,评估转染效率。将使用Attractene Reagent的效率设置为100%,转染效率以与其的相对值表示。
Healthy Cells after Transfection Using Attractene Reagent
使用Attractene Reagent转染后的健康细胞。
使用Attractene Transfection Reagent或另一家供应商提供的Reagent L,参照生产商的说明将DNA(pGFP)转染至HepG2细胞。FACS分析证明,在两种培养物中含有相同数量的转染细胞。转染后两天,采用光学显微镜观察细胞。[A] 使用Attractene Reagent转染的细胞健康且有活性。[B] 而使用Reagent L转染的细胞的死亡率较高。[C] 使用CellTiter-Blue Assay (Promega) 检测细胞活性。使用Reagent L转染的细胞的活性明显低于使用Attractene Reagent转染的细胞(设置为100%)。
Flexible, Rapid Fast-Forward Protocols
灵活且快速的Fast-Forward实验方案。
传统上,在转染前一天接种细胞。使用Fast-Forward实验方案,可在转染当天接种细胞,节省了时间和精力。
Effective Knockdown after shRNA Vector Transfection
shRNA载体转染后的高效敲除。
[A] 仅将表达绿色荧光蛋白(pGFP)的质粒转染至HEK293细胞,或者将[B] pGFP和表达针对绿色荧光蛋白基因的shRNA的质粒(pGFP + pshGFP)共转染至HEK293细胞,或者将[C] pGFP和表达scrambled shRNA的阴性对照质粒(pGFP + pNegative)共转染至HEK293细胞。在完成pGFP及shRNA载体pshGFP共转染后,绿色荧光蛋白被高效沉默,说明共转染高效完成。