Taq PCR Master Mix Kit

For convenient PCR setup using a premixed solution

  • Minimal optimization due to QIAGEN PCR Buffer
  • Master mix format for easy reaction setup
  • Fewer pipetting steps minimize the risk of contamination
Taq PCR Master Mix contains Taq DNA Polymerase, the unique QIAGEN PCR Buffer that minimizes the requirement for optimization, and dNTPs. The convenient 2x master mix format reduces pipetting steps, increasing throughput and reproducibility, while reducing the risk of contamination.
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Taq PCR Master Mix Kit (250 U)
3 x 1.7 ml Taq PCR Master Mix containing 250 units Taq DNA Polymerase, 3 x 1.7 ml Distilled water
 201443
195,00 €
Taq PCR Master Mix Kit (1000 U)
12 x 1.7 ml Taq PCR Master Mix containing 1000 units Taq DNA Polymerase, 12 x 1.7 ml Distilled water
 201445
661,00 €
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The Taq PCR Master Mix Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Reproducible PCR.|Tolerance to variable magnesium concentration.|Tolerance of different primer Tm values.|Lot-to-lot reproducibility.|Specific amplification of long PCR products.|Increased specificity of primer annealing.|
A fragment of the hepatitis B surface antigen gene (gene S) was amplified from 10, 20, and 50 copies of target template, using the Taq PCR Master Mix Kit. Five parallel amplifications were performed for each amount of starting template DNA. Equal volumes of the PCR products were analyzed on a 2% agarose gel. C: negative control; M: markers.|PCR amplification was performed with the QIAGEN PCR Buffer and Taq DNA Polymerase (QIAGEN), using the indicated Mg2+ concentrations. The same PCR was performed in parallel using a PCR buffer and Taq  polymerase from another supplier (Supplier AII). The single-copy human prion protein gene was amplified successfully in each case using the buffer and enzyme from QIAGEN. M: markers.|The human single-copy cystic fibrosis gene was amplified with Taq DNA Polymerase and the QIAGEN PCR Buffer using the indicated annealing temperatures. Primers employed were a 22mer with a Tm of 57.5°C (GC content: 54.5%) and a 32mer with a Tm of 85.2°C (GC content: 78%).  For analysis, 10% of a 100 µl reaction was loaded on an agarose gel. M: markers.|A fragment of the single-copy gene for cystic fibrosis was amplified from 30 ng, 3 ng, and 300 pg human genomic DNA corresponding to 104, 103, and 102 copies of target template, respectively. Three different lots of QIAGEN's Taq DNA Polymerase were used and equal volumes of the PCR product were analyzed on a 1% agarose gel. M: markers.|Three different-sized products from human genomic DNA were amplified using eitherTaq DNA Polymerase and PCR Buffer (QIAGEN), or a polymerase and buffer from another supplier (Supplier AII). For analysis, 10% of each reaction was loaded on the gel. Results from duplicate PCR amplifications are shown. M: markers.|Ammonium and potassium cations in QIAGEN PCR Buffers increase specificity of primer annealing. K+ binds to the phosphate groups (P–) on the DNA backbone, stabilizing the annealing of the primers to the template. NH4+, which exists both as the ammonium ion and as ammonia under thermal-cycling conditions, can interact with the hydrogen bonds between the bases (B), destabilizing the weak hydrogen bonds at mismatched bases. The combined effect of the two cations maintains the high ratio of specific-to-nonspecific primer-template binding over a wide temperature range.|
Performance
TheTaq PCR Master Mix Kit outperformed kits tested from other suppliers and ensures reliable PCR performance in a wide range of PCR applications — without the need for time-consuming optimization (see figure "Reproducible PCR"). Taq DNA Polymerase ensures highly specific amplification with different primer–template systems (see figures "Tolerance of different primer Tm values" and "Specific amplification of long PCR products"). Every lot of QIAGEN's Taq DNA Polymerase is subjected to a comprehensive range of quality control tests, including a stringent PCR specificity and reproducibility assay in which low-copy targets are amplified from human genomic DNA (see figure "Lot-to-lot reproducibility"). Due to the unique PCR buffer included in the master mix, optimization of PCR by varying the annealing temperature or the Mg2+ concentration is dramatically reduced and often not required (see figures "Wide annealing temperature window" and "Tolerance to variable magnesium concentration").

Taq DNA Polymerase specifications

Concentration: 5 units/µl
Recombinant enzyme: Yes
Substrate analogs: dNTP, ddNTP, dUTP, biotin-11-dUTP, DIG-11-dUTP, fluorescent-dNTP/ddNTP
Extension rate: 2–4 kb/min at 72°C
Half-life: 10 min at 97°C; 60 min at 94°C
Amplification efficiency: ≥105 fold
5'–>3' exonuclease activity: Yes
Extra A addition: Yes
3'–>5' exonuclease activity: No
Contaminating nucleases: No
Contaminating RNases: No
Contaminating proteases: No
Self-priming activity: No

Principle

The Taq PCR Master Mix Kit includes QIAGEN's Taq DNA Polymerase in a premixed format. This ready-to-use solution also includes the QIAGEN PCR Buffer, MgCl2, and ultrapure dNTPs at optimized concentrations. Only primers and template DNA need to be added to set up PCR. Due to the convenient master mix format, pipetting errors are minimized, ensuring highly reproducible PCR results (see figure "Reproducible PCR"). Taq PCR Master Mix can be stored at 2–8°C for up to 2 months , allowing even faster PCR setup by eliminating thawing time.

Taq DNA Polymerase

Taq DNA Polymerase DNA Polymerase is a high-quality recombinant enzyme that is suitable for general and specialized PCR applications (see figures "Tolerance of different primer Tm values" and "Specific amplification of long PCR products").

QIAGEN PCR Buffer 

The innovative QIAGEN PCR Buffer has been developed to save time and effort by reducing the need for PCR optimization. QIAGEN PCR Buffer contains both KCl and (NH4)2SO4 (see figure "Increased specificity of primer annealing"). This unique buffer facilitates the amplification of specific PCR  products. During the annealing step of every PCR cycle, the buffer allows a high ratio of specific-to-nonspecific primer binding. Owing to a uniquely balanced combination of KCl and (NH4)2SO4, the PCR buffer provides stringent primer-annealing conditions over a wider range of annealing temperatures and Mg2+ concentrations than conventional PCR buffers. Optimization of PCR by varying the annealing temperature or the Mg2+ concentration is dramatically reduced and often not required (see figures "Wide annealing temperature window" and "Tolerance to variable magnesium concentration").

Procedure
Taq PCR Master Mix includesTaq DNA Polymerase, QIAGEN PCR Buffer, MgCl2, and ultrapure dNTPs at optimized concentrations. The ready-to-use 2x master mix format minimizes pipetting errors and also provides greater convenience. PCR setup is fast, easy, and straightforward — only primers and template DNA need to be added. The easy-to-follow protocol provided with the kit ensures specific amplification and PCR success at the first attempt.
Applications

The Taq PCR Master Mix Kit is used for standard and specialized applications, including:

  • General PCR
  • RT-PCR
  • Screening
  • PCR-based DNA fingerprinting (VNTR, STR, and RAPD)
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Feature
Specifications
Applications PCR, RT-PCR, DNA fingerprinting
dNTP's included Yes (in Master Mix)
Enzyme activity 5' -> 3' exonuclease activity
Mastermix Yes
Reaction type PCR amplification
Real-time or endpoint Endpoint
Sample/target type Genomic DNA and cDNA
Single or multiplex Single
With/without hotstart Without hotstart

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Kit Handbooks
1
Taq PCR Handbook - (EN)
For standard and specialized PCR applications with minimal optimization
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References
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