Investigator DIPplex Kit
For multiplex amplification of 30 deletion/insertion polymorphisms plus Amelogenin
The Investigator DIPplex Kit enables the amplification of 30 deletion/insertion polymorphisms (also known as INDELs) plus Amelogenin. This provides an equally sensitive alternative to STR- and SNP-based human identification. The kit is designed to analyze highly degraded DNA, thanks to the maximum amplicon size of just ~160 bp. The genetic analysis is calibrated with BT5 Matrix, an optimized dye technology.
Performance
The Investigator DIPplex Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode), and the ABI PRISM 310, ABI PRISM 3100, and Applied Biosystems 3130 Genetic Analyzers. The recommended amount of DNA under standard conditions is 0.35 ng. Internal validations demonstrated robust and balanced results with 0.2–0.5 ng, and reliable results with <0.1 ng DNA. The Investigator DIPplex Kit has significant advantages over standard STR- and SNP-based DNA profile generation, including the absence of stutter peaks and the very short amplicons (under 160 bp).
Principle
Human identification is commonly based on the analysis of short tandem repeats (STRs) or single nucleotide polymorphisms (SNPs), depending on the demands of an examination or on the sample quality. The Investigator DIPplex Kit uses an alternative means of human identification by allowing the analysis of biallelic deletion/insertion polymorphisms (DIPs, also called INDELs).
Procedure
The 30 biallelic DIP loci plus Amelogenin are simultaneously amplified in a single PCR and labeled with four dyes. See the table "Technical specifications" for more information.
Applications
The Investigator DIPplex Kit is for generation of DNA profiles from forensic stains, hair, and bone samples, and it is highly suitable for:
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