mericon RR Soy Kit
For real-time PCR detection of Roundup Ready soy in food or animal feed
The mericon RR Soy Kit is designed for the target-specific detection of Roundup Ready soy in food, animal feed, and pharmaceutical products. The assay uses a real-time PCR-based protocol and forms part of the comprehensive QIAGEN food testing portfolio, which also features dedicated sample preparation kits and assays for pathogen detection and ingredient authentication. The mericon RR Soy Kit performs optimally on the Rotor-Gene Q, but has also been validated for block thermal cyclers.
The mericon RR Soy Kit is intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Performance
The mericon RR Soy Kit is designed for the detection of Roundup Ready soy in food and animal feed. The mericon RR Soy Kit can detect as little as 10 copies of target DNA in a reaction and exhibits high specificity for Roundup Ready soy . No cross-reactivity was observed with other plant or animal species, GMOs or genetic constructs not found in Roundup Ready soy using 50 ng of tested DNA (see the table "Results of mericon RR Soy Kit cross-reactivity study"). Results of mericon RR Soy Kit cross-reactivity study
Principle
The mericon Roundup Ready Soy Kit is designed for the qualitative detection of the genetically modified organism (GMO) Roundup Ready soy in food and animal feed samples. The genetic modification in the RR soy line provides resistance to the active compound glyphosate in herbicides, which is detected by the mericon RR Soy Kit. RR soy is the most frequently used genetically modified soy line. Detection of GMOs using real-time PCR is based on the amplification of a specific region of the relevant GMO genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward. mericon PCR Assays include an internal control to control potential inhibition. This aids in the straightforward interpretation of the results (see the table "Summary of possible PCR outcomes"). The PCR primer set for each assay is highly specific and targets either a genetic construct that is commonly used in GMOs or a DNA sequence that is specific for a particular GMO. These targets were verified bioinformatically and experimentally.
Procedure
DNA is extracted from a sample of food or animal feed using a dedicated sample preparation kit. QIAGEN's DNeasy mericon Food Kit is suitable for a wide range of food matrices, including those with high inhibitor contents. The isolated, pure DNA is assayed using a streamlined real-time PCR protocol that returns results in 82 minutes on QIAGEN's Rotor-Gene Q. The protocol has also been validated for use on other thermal block cyclers. The results are displayed as easy-to-interpret PCR curves.
Applications
The mericon RR Soy Kit is for real-time PCR-based detection of Roundup Ready soy in food, animal feed, and pharmaceutical products.
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