Can the Rotor-Gene Q perform gradient PCR?
FAQ ID -3183

No, it cannot do gradient PCR.  For gradient PCR, one needs a heatblock cycler where different areas of the heatblock have different temperatures.  Since the Rotor-Gene Q uses a heat chamber and a rotor, one cannot specifically set different temperatures for sections of the rotor. Therefore, we recommend touchdown PCR to determine optimal annealing temperatures.