miRNA detection using qPCR with LNA technology

Mar 28, 2018 1:00 PM–2:00 PM (EST)
Duration: 1hrs

miRNAs are naturally occurring, noncoding RNAs that mediate post-transcriptional gene regulation. miRNAs are small, around 22 nucleotides long, but mighty. Serving as regulators of gene expression with an important role in many biological processes, miRNAs are broadly studied. There are many tools and methods available to support miRNA research – choosing the right tools for your miRNA experiments is essential for success and bringing you closer to your next big discovery.

Part 1: Advancing miRNA research – best practices for your experiments
This webinar will guide you through the steps of miRNA experiments. We will explain best practices and tools to consider at each step, and the pros and cons of the different assay methods. We will also explain how to isolate miRNA from different sample types.

Part 2: Introduction to miRNA discovery using NGS
In this webinar, we will introduce the QIAseq miRNA Library Kit which features unique molecular indexing (UMIs) and a gel-free workflow from as little as 1ng of total RNA.  Whether you’re working with serum, cells, tissues or FFPE samples, QIAGEN’s LNA-enhanced chemistry and proprietary technology ensures higher levels of mapped miRNA reads. Cloud-based NGS data analysis allows anyone with access to an NGS instrument to go from sample to data in as little as 3 days.  Come join us as we go through best-practices for miRNAseq library construction, and data analysis.

Part 3: Successfully detect miRNAs using qPCR with LNA technology
This webinar will introduce the advantages of miRNA detection using qPCR with LNA (locked nucleic acids) technology. In addition, we will discuss how to overcome the challenges of analyzing biofluids such as plasma.

Dr. Francesca Di Pasquale

Francesca Di Pasquale
Dr. Francesca Di Pasquale obtained her degree in medicinal chemistry in 2004 from the University of Padova in Italy. During this time she also studied at the University of Medicinal Chemistry in Bonn in Germany, writing her diploma thesis at the Institute of Pharmacology and Toxicology. She then joined the Faculty of Chemistry and Life Science at the Excellence University of Konstanz, obtaining a PhD in Natural Science in 2008. She now holds the position at QIAGEN of Senior Scientist in Applied Testing R&D.