Comparing DNA acquired from 0.25 grams of garden soil pre- and post-cleanup reveals high levels of contaminants before cleanup occurs. UV-absorbing contaminants skew DNA concentrations in a sample, as seen in the "Garden soil, no inhibitor removal" curves. UV-absorbing contaminants were removed from the DNA of the contaminated material technical replicates, using the MagAttract PowerClean DNA Kit.
A) Samples of garden soil were analyzed with and without inhibitor removal using a Qubit assay showing greater than 90% of the original input material. Note: NanoDrop analysis, as seen in Figure 1, has higher DNA concentrations due to overestimation common in NanoDrop analyses. B) Replicates processed with the MagAttract PowerClean DNA Kit show retention of dsDNA integrity throughout the cleanup process. (1.2% agarose gel).
PCR inhibitors were removed from garden soil DNA replicates using the MagAttract PowerClean DNA Kit, as shown in the results of the 16s qPCR amplification analysis. Comparitively, pre-cleanup DNA only mildly responded to amplification after a 1:10 dilution.