EpiTect ChIP OneDay Kit
For fast and effective chromatin immunoprecipitation
The EpiTect ChIP OneDay Kit is designed for performing chromatin immunoprecipitation (ChIP) in 6 hours with a ChIP-grade antibody.
The EpiTect ChIP OneDay Kit isolates DNA with high yield and high purity, free from inhibitors. As a result, real-time PCR analysis of the DNA provides highly reproducible results and good signal-to-noise ratios. See figures "A high degree of reproducibility", "Good signal-to-noise ratios", and "DNA free from PCR inhibitors".
The EpiTect ChIP OneDay Kit is designed for performing chromatin immunoprecipitation (ChIP) using chosen antibodies to specifically enrich genomic DNA fragments bound in vivo to transcription factors, modified histones, or other nuclear factors of interest.
The EpiTect ChIP OneDay Kit decreases the time required for cross-link reversal and DNA isolation down to 1 hour versus traditional ChIP methods that require 6–8 hours. This time saving allows the rapid quality control and assessment of the size of sheared chromatin before immunoprecipitation, and enables more reliable subsequent real-time PCR detection.
It takes just 6 hours to generate a high yield of enriched ChIP DNA that is free of inhibitors and ready for real-time PCR quantification (see flowchart "EpiTect ChIP OneDay protocol"). The kit includes all the buffers and reagents needed for the ChIP preparative process, except for cross-linking agent and antibodies.
Using the EpiTect ChIP OneDay Kit, chromatin from cells or tissues is first sheared and then incubated and immunoprecipitated with ChIP-grade antibodies from an EpiTect ChIP Antibody Kit or other sources. These antibodies enable precipitation of chromatin associated with the protein of interest. After purifying the ChIP DNA fraction with the EpiTect ChIP OneDay Kit, the amount of DNA enriched by the antibody of interest can be quantified by real-time PCR using EpiTect ChIP qPCR Assays or EpiTect ChIP qPCR Arrays.
Among other applications, the EpiTect ChIP OneDay Kit, used with the EpiTect ChIP Antibody Kit and EpiTect ChIP qPCR Arrays and Assays, streamlines:
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