MagAttract DNA Blood Midi M48 Kit
For automated purification of genomic DNA from blood or buffy coat samples using the BioRobot M48
The MagAttract DNA Blood Midi M48 Kit provides automated purification of genomic DNA on the BioRobot M48 using proven MagAttract magnetic particle technology. DNA purification and magnetic separation take place in the pipet tips, increasing the efficiency of the procedure. Up to 48 samples can be processed per run in increments of 6 samples. Variable elution volumes enable easy optimization of DNA concentration.
The MagAttract DNA Blood Midi M48 Kit provides fully automated purification of DNA (see figure "Reproducible purification of high-quality genomic DNA") and the purified DNA is free of PCR inhibitors (see figure "Reproducible performance in sensitive PCR analysis"). The MagAttract DNA Blood Midi M48 Kit is suitable for use with ACD-, heparin-, and EDTA-preserved whole blood samples (see figure "High-quality genomic DNA from differently preserved whole-blood samples").
The MagAttract DNA Blood Midi M48 Kit is designed for fully automated DNA purification. Proven MagAttract magnetic particle technology in combination with the BioRobot M48 workstation provides high-quality DNA, suitable for direct use in sensitive downstream applications. Advantages of the system include scalable sample volumes and elution volumes (see table), giving the yield and concentration of high-quality DNA appropriate for the intended downstream application.
MagAttract technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. Optimized buffers lyse samples, and DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt (see flowchart "MagAttract DNA Blood Midi M48 procedure"). DNA bound to the magnetic particles is then efficiently washed. Two different wash buffers are used, followed by a rapid rinse with distilled water, which considerably improves the purity of the DNA. High-quality DNA is eluted in the water provided.
DNA purified using the MagAttract DNA Blood Midi M48 Kit can be used in a wide range of downstream applications, including:
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