Supporting antimicrobial resistance research for known and future threats – QIAGEN scientists share their expertise
Webinar part 1 — Challenges in extracting nucleic acid for antimicrobial resistance monitoring
Sample types commonly used for monitoring antimicrobial resistance, such as wastewater and stool, present the challenge of extracting pure nucleic acid while ensuring complete representation of the microbial species and capturing the antimicrobial gene. In this webinar, we present methods and protocols for optimizing the informational content that can be extracted from even these most challenging samples.
Webinar part 2 — NGS in Antimicrobial Research: Tips & Tricks
Antimicrobial resistance (AMR) research uses many types of sample types, some of which present challenges when using NGS, particularly for getting the high-quality, useful and actionable insights. In this webinar, we will discuss:
- Tips and tricks for NGS of difficult sample types (e.g., wastewater and stool)
- The pros and cons of targeted vs metagenomic NGS approaches
- Novel methods for AMR research
Webinar part 3 — AMR monitoring with nanoplate digital PCR
Digital PCR, with its accurate absolute quantification, provides a suitable method for specific and rapid monitoring of AMR genes in various sample types.
In this webinar, we demonstrate how dPCR based on nanoplates enables rapid profiling and identification of AMR genes in samples from wastewater, the human microbiome and other environmental samples. Guidance will be given on how to transition from real-time PCR to dPCR and how to establish the technology in a research lab.