Accurate NGS library quantification using nanoplate digital PCR
Accurate quantification of next-generation sequencing (NGS) libraries is a prerequisite for optimal yield on Illumina NGS platforms. Absolute quantification of target molecules by digital PCR enables more accurate measurement of these libraries, resulting in efficient use of NGS flow cells.
In this webinar, we will introduce the QIAcuity dPCR workflow for quantifying Illumina NGS libraries using a library-specific primer assay in combination with the QIAcuity EG PCR Kit. We will showcase how to use the dPCR system to accurately quantify NGS libraries regardless of their average fragment length and composition.
Key learning objectives:
- How to set up a dPCR reaction for NGS library quantification
- What level of performance can you expect from dPCR for NGS library quantification
- How quantification with the QIAcuity compares to standard methods