Epigenetics

Genetic alterations do not represent the full picture of gene expression or cellular function changes that are observable during oncogenesis. Epigenetic mechanisms influence gene activity without changing the DNA sequence and include DNA methylation, which is associated with gene silencing of tumor suppressor or DNA repair genes. DNA methylation is a stable, heritable, covalent modification to DNA, occurring mainly at CpG dinucleotides, but is also found at non-CpG sites. Interrogating DNA methylation degree can be challenging in FFPE and liquid biopsy samples, amplifying the need for robust, reliable solutions that work with low-input samples. QIAGEN’s Sample to Insight solutions for epigenetics applications address these challenges to deliver reliable data and powerful insights.

What’s new in the world of epigenetics?

Access the latest methylation analysis resources at your fingertips!

Hypomethylation vs. hypermethylation

Targeted methylation sequencing

Fastest workflow with the lowest sample input for methylation analysis

High performance from tissue, FFPE and liquid biopsy samples with just 1–10 ng of DNA input
Go from DNA to data analysis in one day
Integrated analysis workflows for the non-bioinformatics researcher
Flexibility to customize or fully design panels to your project

Are you seeing a true variant or a false positive?

Each bisulfite-treated DNA molecule is tagged with a unique molecular index (UMI) before amplification. During analysis, PCR duplicates and false positives are removed to give an accurate methylation profile.

Can you cover every region, even regions with high GC-content?

Single primer extension technology (SPE) uses only one specific primer plus a universal primer for each amplicon, compared to the traditional 2-primer approach.

This means:

Lower risk of primer dimers and dropouts
Higher uniformity
Higher library complexity
Only a single pool of primers required

Scaling up your targeted panel content? SPE doesn’t require a primer redesign!

From gDNA to methylome insights in one tube and one day

Save precious samples: Low-input volume requirement safeguards limited samples
Save time: Go from genomic DNA to NGS library in a day using the single-tube protocol
Trust results: Highly specific and uniform bisulfite treatment to convert unmethylated cytosines to uracil
Integrated data analysis: Pre-made and customizable bioinformatics workflows for every researcher

Explore our QIAseq Targeted Methyl and Custom Panels.

Our Illumina-compatible panels include:

Human Breast Cancer Panel
Human Colorectal Cancer Panel
Immuno-Oncology Panel
Human T-cell Infiltration Panel

Fully automate your Pyrosequencing

Pyrosequencing integrates sequence detection and quantification and plays a vital role in research applications looking at epigenetic DNA methylation in gene expression regulation. Discover an easy-to-use, automated protocol that prepares single-stranded DNA samples without manual interaction from the user.

AUTOMATE YOUR WORKFLOW

Watch the latest webinars on methylation analysis

Unlock liquid biopsy samples with NGS-based methylation detection from 10 ng DNA
In this new webinar, we introduce a novel, highly sensitive and faster NGS-based approach to targeted methylation detection using the QIAseq Targeted and Custom Methyl Panels.
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Streamlining analysis of targeted NGS methylation data using QIAGEN CLC Genomics Workbench software
In this webinar, we describe additional aspects of our Sample to Insight solution, focusing on the analysis and visualization of targeted methyl panel data using QIAGEN CLC Genomics Workbench software.
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All photos taken prior to COVID-19

Next-generation sequencing