EpiTect ChIP Antibody Kit
For analysis of chromatin immunoprecipitated DNA
The EpiTect ChIP Antibody Kit provides the specific antibodies, control IgGs, plus positive and negative control qPCR primers for a complete and reliable analysis of chromatin immunoprecipitated DNA.
Principle
EpiTect ChIP Antibody Kits are available for transcription factors, modified or unmodified histones, and other nuclear factors. Both the ChIP-grade antibodies and control IgGs have been rigorously verified for their specificity and efficiency in chromatin IP experiments using different chromatin sources. The EpiTect ChIP Antibody Kit includes positive and negative control qPCR primers, ensuring the accuracy of ChIP analysis. The EpiTect ChIP Antibody Kit yields very high signal-to-noise ratios when combined with the EpiTect ChIP OneDay Kit. Available EpiTect ChIP Antibody Kits include:
Procedure
Using the EpiTect ChIP OneDay Kit, chromatin from cells or tissues is sheared and then incubated and immunoprecipitated with ChIP-grade antibodies from the EpiTect ChIP Antibody Kit. These antibodies enable precipitation of chromatin associated with the protein of interest. After purifying the ChIP DNA fractions with the EpiTect ChIP OneDay Kit, the amount of DNA enriched by the antibody of interest can be quantified by real-time PCR using EpiTect ChIP qPCR Assays or EpiTect ChIP qPCR Arrays.
Applications
The EpiTect ChIP Antibody Kit is highly suited for the analysis of chromatin immunoprecipitated DNA. Among other applications, the EpiTect ChIP Antibody Kit, when used with the EpiTect ChIP OneDay Kit and EpiTect ChIP qPCR Arrays or Assays, streamlines:
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