mericon Shigella spp Kit
For real-time PCR detection of Shigella subspecies in food or animal feed
The mericon Shigella spp Kit is designed for the target-specific detection of Shigella species DNA in food, animal feed, and pharmaceutical products. The assay uses a real-time PCR-based protocol and forms part of the comprehensive QIAGEN food testing portfolio, which also features dedicated sample preparation kits and assays for ingredient authentication and GMO DNA detection. The mericon Shigella spp assay performs optimally on the Rotor-Gene Q, but has also been validated for block thermal cyclers.
The mericon Shigella spp Kit is intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. This product is not intended for the diagnosis, prevention, or treatment of a disease.
The mericon Shigella spp Kit is for the specific detection of several Shigella species in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Shigella DNA in a reaction and it exhibits high specificity for pathogens of this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Shigella spp Kit cross-reactivity study").
Results of mericon Shigella spp Kit cross-reactivity study
The mericon Shigella spp Kit is designed for the qualitative detection of several Shigella species in food and animal feed samples after enrichment. Shigella is only found in humans and apes. Some Shigella species are able to produce enterotoxins and shiga toxins. The bacteria infect their host by fecal-oral transmission, whichmost commonly leads to symptoms of diarrhea, fever, and stomach cramps.
Detection of pathogens using real-time PCR is based on the amplification of a specific region of the relevant pathogen genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward.
mericon PCR Assays include an internal control to control potential inhibition. This aids in the straightforward interpretation of the results (see the table "Summary of possible PCR outcomes").
The PCR primer set for each assay is highly specific and targets a unique and conserved region of the tested pathogen genome that has been bioinformatically and experimentally verified.
DNA is extracted from an enrichment culture of a sample of food or animal feed using a dedicated sample preparation kit. The mericon Bacteria Kit is for the isolation of bacterial DNA from Gram-negative bacteria. The mericon Bacteria Plus Kit is for the isolation of bacterial DNA from Gram-positive bacteria.
The isolated, pure bacterial DNA is assayed using a streamlined real-time PCR protocol that returns results in 73 minutes on QIAGEN's Rotor-Gene Q. The protocol has also been validated for use on other thermal block cyclers.
The results are displayed as easy-to-interpret PCR curves.
The mericon Shigella spp Kit is for real-time PCR-based detection of Shigella species in food, animal feed, and pharmaceutical products.
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