Do I need the sequence specific miRNA primers in the RT step of the miRNA qPCR Assays?
FAQ ID -2725

No. Because the miScript miRNA qPCR assay technology uses universal reverse transcription in the RT step, sequence specific miRNA primers are not needed in this step. Therefore, cDNA obtained from universal RT can be used for any miScript miRNA expression analysis qPCR assay or PCR array.