Small-scale plasmid purification — 96-well format
| DNA grade |
Transfection grade |
Molecular biology grade |
Sequencing grade |
|
|---|---|---|---|---|
| Speed | Standard | Fast | Fast | Standard |
| Kit name | QIAGEN Plasmid Plus 96 Miniprep and BioRobot Kits |
QIAprep 96 Plus Miniprep and BioRobot Kits |
QIAprep 96 Turbo Miniprep and BioRobot Kits |
R.E.A.L. Prep 96 Plasmid Kit |
| Binding capacity |
<50 µg | <50 µg | <20 µg | <30 µg |
| Culture volume (3–4 x 109 cells/ml) |
1.25 –5 ml | 1.25 –5 ml | 1–5 ml | 2.5 ml BAC/PAC/P1, 1.3 ml plasmid, 1.3–2.5 ml cosmid |
| Yield | 15–50 µg | 15–50 µg | 4–20 µg | 1 µg BAC/PAC/P1 |
| Elution volume |
80 µl (100–120 µl for a 5 ml culture) |
80 µl (100–120 µl for a 5 ml culture) |
100 µl | 22 µl BAC/PAC/P1, 50–250 µl plasmid, 50 µl cosmid |
| Procedure | QIAvac 96, centrifuge, BioRobot Universal System |
QIAvac 96, centrifuge, BioRobot Universal System |
QIAvac 96, centrifuge, BioRobot Universal System |
QIAvac 96, centrifuge |
| Time | 50 min | 40 min | 45 min | 70 min |
Small-scale plasmid purification kits
| DNA grade | Transfection grade |
Molecular biology grade |
Sequencing grade |
|---|---|---|---|
| Speed | Fast | Fast | Ultrafast |
| Kit name | QIAGEN Plasmid Mini Kit |
QIAprep Spin Miniprep Kit |
Quicklyse Miniprep Kit |
| Binding capacity |
<20 µg | <50 µg | <15 µg |
| Culture volume (3–4 x 109 cells/ml) |
3 ml | 1–5 ml | 1–3 ml |
| Yield | 20 µg | 5–50 µg | 8–15 µg |
| Elution volume | Variable, 50 µl recommended |
50 µl | 50 µl |
| Procedure | Centrifuge, QIAcube |
QIAvac 24 Plus, centrifuge, QIAcube |
Centrifuge |
| Time | 80 min | 30 min | 9 min |
Large-scale plasmid purification kits
|
DNA grade |
Endotoxin-free | Transfection grade | Molecular biology grade | |||||
|---|---|---|---|---|---|---|---|---|
| Speed | Standard | Standard | Fast |
Ultrafast | Ultrafast | Standard | Ultrafast | |
| Kit name | EndoFree Plasmid Kits |
QIAGEN Plasmid Kits | QIAfilter Plasmid Kits |
QIAGEN Plasmid Plus Kits |
HiSpeed Plasmid Kits |
QIAGEN Large-Construct Kit |
CompactPrep Plasmid Kits |
|
| Culture volume (3–4 x 109 cells/ml) |
Midi | _ |
25 ml, 100 ml |
25 ml, 50–100 ml |
20–25 ml, 25–35 ml (hy), 50 ml |
50 ml, 150 ml |
500 ml | 25 ml, 50 ml |
| Maxi | 100 ml, 250 ml |
100 ml, 500 ml |
100 ml, 250 ml |
80–100 ml, 100–130 ml (hy), 200 ml |
150 ml, 250 ml |
100 ml, 250 ml |
||
| Mega | 500 ml/1.5 g, 2.5 l / 7.5g |
500 ml/1.5 g, 2.5 l/ 7.5g |
500 ml/1.5 g, 2.5 l/7.5g |
500 ml |
500 ml only rec for hc |
– | ||
| Giga | 2.5 l/7.5 g, not rec. for lc |
2.5 l/7.5 g, 5 l/15 g |
2.5 l/7.5 g, not rec. for lc |
2.5 l |
2.5 l only rec for hc |
– | ||
| Yield | Midi | – |
75–100 µg, 20–100 µg |
75–100 µg, 20–100 µg |
100–200 µg, 150–250 µg (hy), 30–100 µg |
100–200 µg, 30–100 µg |
Up to 50 µg BAC/PAC/P1 DNA, up to 200 µg cosmid DNA |
100–200 µg, 30–100 µg |
| Maxi | 300–500 µg, 50–250 µg |
100–500 µg, 20–250 µg |
300–500 µg, 50–250 µg |
750 µg, 1 mg (hy), 50–250 µg |
300–750 µg, 50–250 µg |
350–750 µg, 50–250 µg |
||
| Mega | 1.5–2.5 mg, 0.5–2.5 mg |
1.5–2.5 mg, 0.5–2.5 mg |
1.5–2.5 mg, 0.5–2.5 mg |
Up to 2.5 mg |
1.5–2.5 mg, endotoxin-free <0.1 EU/µg DNA |
– | ||
| Giga | 7.5–10 mg |
7.5–10 mg, 1–5 mg |
7.5–10 mg |
Up to 10 mg |
7–10 mg, endotoxin-free <0.1 EU/µg DNA |
– | ||
| Time | Midi | – |
150 min |
110 min |
20 min |
<45 min |
280 min | <20 min |
| Maxi | 150 min |
160 min |
120 min |
20 min |
<60 min |
<20 min | ||
| Mega | 220 min |
220 min |
190 min |
40 min |
50 min |
– | ||
| Giga | 310 min |
320 min |
280 min |
50 min |
50 min |
– | ||
| Procedure | Lysate clearing by precipitator or vacuum, gravity-flow |
Gravity-flow | Lysate clearing by precipitator or vacuum, gravity-flow |
Vacuum and centriguge |
Gravity-flow and precipitator |
Centrifuge | Centrifuge and vacuum |
|