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DNA Sequencing

DNA sequencing

The workflow for next-generation DNA sequencing is as follows:

  • DNA sample preparation
  • Library construction and validation
  • Massive parallel clonal amplification of library molecules
  • Sequencing
Next DNA sample QC for NGS
Previous NGS sequencers
    • NGS sequencers
    • DNA sequencing
    • DNA sample QC for NGS
      • Gel electrophoresis
      • Spectrophotometry
      • Real-time PCR
    • Library preparation
    • Library QC for NGS
      • Assessment of library size
      • Determination of library quantity
    • Metagenomics
    • RNA sequencing
      • Factors to consider in RNA-seq
      • RNA-seq procedure
    • Gene-regulation studies
      • ChIP-Seq
    • References