Get the expertise and proven technologies to be confident of the sequence of every transcript in your sample
Whole Transcriptome Seq Service enables the characterization of all RNA transcripts for a given organism, including coding and non-coding RNA (above 170 nt in size), regardless of whether or not they are polyadenylated. Libraries are generated according to the type of sequencing. We perform ribosomal RNA depletion (through the use of a biotin–streptavidin bead-based system) for whole transcriptome sequencing. The resulting RNA is fragmented and converted into whole transcriptome cDNA NGS libraries.
Whole transcriptome paired-end sequencing is recommended for both discovery work and differential expression analysis. By using paired-end sequencing, it is also possible to identify splice variants with higher confidence.
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