PCR/qPCR/dPCR
PCR sensible, especÃfica, robusta y fiable
El éxito de los experimentos de amplificación génica depende de varios factores crÃticos, como el rendimiento, la sensibilidad del ensayo, la robustez de la reacción incluso con moldes difÃciles, y la normalización fiable de los datos. También puede ser necesario superar los retos especÃficos asociados al método de PCR que se utilice.
Elija el método que elija, ofrecemos numerosas soluciones optimizadas para PCR convencional, qPCR y dPCR. Nuestros kits, ensayos, instrumentos y accesorios le ayudarán a amplificar y detectar dianas con confianza y a optimizar el flujo de trabajo de prácticamente cualquier aplicación.
- Measure gene expression (mRNA, miRNA)
- Quantify DNA or RNA targets
- Detect & quantify pathogen and/or viral RNA/DNA
- Quantify residual DNA
- Convert RNA to cDNA for quantification
- Mutation detection (including rare/ultra-rare variants)
- Analyze copy number variation (CNV)
- Detect viral load
- Gene expression quantification
- Quantify low-abundance targets
- Measure residual DNA
- Amplify DNA for cloning or sequencing
- Qualitative pathogen detection (presence/absence)
- CRISPR screening
- Genotyping
- Convert RNA to cDNA for storage
- Cq/Ct values
- Relative quantification (ΔΔCq) or absolute quantification from standards
- cDNA for downstream use
- Absolute target quantification (copies/µL reaction)
- Fractional abundance (%)
- Mutation/target frequency
- Copy number per genome
- Amplified DNA or cDNA product
- Band visualization on gel
- Presence/absence result
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Some kits support crude samples such as cells, blood, dried blood spots, saliva, buccal swabs, or gargle
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Purified samples recommended
- Isolated DNA or cDNA
- Isolated RNA
- Quantitative real-time PCR
- Reverse transcription
- Amplification and fluorescence-based detection in real-time for quantification
- Quantitative digital PCR
- Partitions the sample into thousands of reactions and uses end-point PCR + Poisson statistics for absolute quantification without a standard curve
- Standard end-point PCR
- DNA amplification and visualization (e.g., gel-based) and downstream applications
- When reliable quantification is required over a wide linear range
- When standard curve-based or relative quantification is sufficient
- When precise quantification is required
- When detecting rare alleles or small fold changes
- When standard curves are undesirable
- When working near the detection limit (low-abundance targets)
- When qualitative detection without quantification is sufficient
- When preparing amplicons for cloning, genotyping, sequencing, or other downstream applications
- Real-time PCR thermal cycler
- Dye-based (e.g., SYBR Green) or probe-based detection
- QIAcuity dPCR platform
- Probe‑based or intercalating dye detection
- Thermal cycler
- Visualization (e.g., via agarose gel electrophoresis)
Examinar PCR/qPCR/dPCR
Productos
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11 productos encontrados
ID SOLUTIONS IDNAPTEX dPCR Assays
For detecting genomic alterations, such as point mutations, deletions and fusions in DNA extracted from liquid and tissue biopsies using multiplex digital PCR for cancer research applications
QIAcuity High Multiplex Probe PCR Kit
Para muestras desafiantes y multiplexado de dPCR de más de cinco dianas
QIAcuity Residual DNA Quantification and Sizing Kits
For quantification and sizing of host cell DNA with higher accuracy and precision using the QIAcuity Digital PCR System
Q-Solution Kit
Este kit incluye Q-Solution y MgCl2 para obtener mejores resultados de las reacciones de PCR con configuraciones más difÃciles.
QIAcuity RCL Quant Kit
For the detection of Replication Competent Lentivirus (RCL) with high accuracy using the QIAcuity digital PCR instruments
QIAprep& Plasmodium Kit
For direct qPCR detection of Plasmodium spp. from dried blood spots or whole blood.
QIAprep& Plasmodium Assays
For distinguishing between Plasmodium falciparum and non-falciparum species, as well as differentiating four additional species
dPCR PanCancer Kits
For detection of multiple hallmark mutations in cancer-relevant genes using the QIAcuity digital PCR instruments
Rotor-Gene Q
For outstanding performance in real-time PCR
QIAGEN Multiplex PCR Plus Kit
For straightforward and successful multiplex PCR in advanced applications
REPLI-g FFPE Kit
For direct whole genome amplification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue
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