PyroMark Q24 Tests

For analyzing sequence variation or methylation of specific genes using the PyroMark Q24 Instrument

Detection and quantification of different mutations in a single run
Flexible software for post-run analysis of adjacent mutations
Quantification of individual or multiple CpG sites in different genes
Estimation of global CpG methylation in defined regions of interest
Sequence context provides built-in control of the assay

PyroMark Q24 Tests are a range of assays suitable for highly reliable, quantitative mutation and CpG methylation analysis and are optimized for use with the PyroMark Q24. Tests designated with 'Q24' are optimized for use with the PyroMark Q24, while others such as PyroMark HFE and PyroMark MTHFR can be used with the PyroMark Q96 ID and PyroMark Q96 MD as well. For more tests for these instruments, see PyroMark Q96 Tests.

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Cat No./ID: 970012

PyroMark Q24 CpG p16 (4 x 24)

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Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region +148 to +182 in exon 1 of the gene

Cat No./ID: 970022

PyroMark Q24 CpG MLH1 (4 x 24)

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Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region –209 to –181 from the transcription start site in the MLH1 gene

Cat No./ID: 970032

PyroMark Q24 CpG MGMT (4 x 24)

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Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region +17 to +39 of exon 1 of the MGMT gene

Cat No./ID: 970042

PyroMark Q24 CpG LINE-1 (4 x 24)

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Methylation detection assay: PCR primers and sequencing primer for quantification of global methylation level in transposable elements

Cat No./ID: 972432

PyroMark MTHFR (96)

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For detection of variants in the MTHFR gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)

Cat No./ID: 972442

PyroMark HFE (96)

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For detection of variants in the HFE gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)

PyroMark Q24 Tests are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

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Principle

PyroMark Q24 CpG LINE-1, PyroMark Q24 CpG MGMT, PyroMark Q24 CpG MLH1, and PyroMark Q24 CpG p16 enable highly reliable methylation results. In addition, a built-in control for bisulfite treatment improves accuracy and reproducibility of the results.

Procedure

Prior to Pyrosequencing, a biotinylated PCR product is generated. Biotinylated PCR products are bound to streptavidin-coated Sepharose beads. The beads are then captured with the vacuum tool on the PyroMark Vacuum Workstation, and throroughly washed, generating single-stranded DNA suitable for sequencing. PyroMark Q24 Tests are predesigned assays for methylation analysis by Pyrosequencing. Each test includes the primers necessary for generating the biotinylated PCR product, as well as the primer for the Pyrosequencing reaction.

Applications

PyroMark Q24 Tests enable highly accurate, quantitative methylation and mutation (or SNP) analysis applicable in several fields of research, including cancer and epigenetics.

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FAQs (18)

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	How accurate and reliable is PyroMark Q24 in mutation analysis? FAQ ID -2094	View
	Can I order the nucleotides from PyroMark Gold Reagents separately? FAQ ID -2827	View
	How many times can vacuum troughs be re-used with the PyroMark Vacuum Preparation Stations? FAQ ID -2848	View
	What is the amplicon length of the PyroMark CpG LINE assay? -1	View
	What region of LINE gets targeted by the assay for PyroMark Q96 MD or PyroMark Q24? -1	View
	How many CpG sites are analyzed by the PyroMark CpG LINE assay? -1	View
	Does the PyroMark CpG LINE assay target mouse transposons as well? -1	View
	Does the PyroMark LINE assay target all LINE sequences? -1	View
	Can the PyroMark Q96 CpG LINE assay be used with an ID system? -1	View
	How many nucleotides of a homopolymer can be resolved in pyrosequencing? FAQ ID -2871	View
	Can unused wells in a pyrosequencing plate be used in the next run? FAQ ID -2872	View
	What is the reason for signals ceasing in the middle of a pyrosequencing run? FAQ ID -2875	View
	How do I reduce background peaks in the pyrosequencing pyrogram? FAQ ID -2877	View
	How do I prevent a drifting baseline in my pyrosequencing pyrogram? FAQ ID -2878	View
	What is the reason for a high substrate peak in the pyrosequencing pyrogram? FAQ ID -2879	View
	What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram? FAQ ID -2881	View
	Which purity grade is recommended for pyrosequencing primers? FAQ ID -2832	View
	Where can I order the Streptavidin Sepharose beads for pyrosequencing? FAQ ID -2850	View

Kit Handbooks (3)

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	(EN) - PyroMark Q24 CpG LINE-1 Handbook	Show details
	(EN) - PyroMark HFE Handbook	Show details
	(EN) - PyroMark MTHFR Handbook	Show details

Brochures & Guides (1)

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	Pyrosequencing – the synergy of sequencing and quantification	Show details

Scientific Posters (1)

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	(EN) - Pyrosequencing and its applications	Show details

Safety Data Sheets (6)

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	MSDS PyroMark Q24 CpG LINE-1 (4 x 24)
	MSDS PyroMark Q24 CpG MGMT (4 x 24)
	MSDS PyroMark Q24 CpG MLH1 (4 x 24)
	MSDS PyroMark Q24 CpG p16 (4 x 24)
	MSDS PyroMark HFE (96)
	MSDS PyroMark MTHFR (96)

References