Cat. No. / ID: 339330
miRCURY LNA miRNA Custom Panels are ready-to-use 96- and 384-well plates containing individual miRCURY LNA miRNA PCR Assays according to your particular requirements. Both forward and reverse PCR amplification primers are miRNA-specific and are optimized with LNA technology, enabling extremely sensitive and specific miRNA quantification with the miRCURY LNA miRNA PCR System. Each well contains sufficient primer set for one 10 µl reaction.
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The miRCURY LNA miRNA PCR System is the only miRNA quantification platform with absolute specificity (see the miRQC study published in Nature Methods) and out-performed another probe-based miRNA qPCR platform in specificity tests (see figure Specificity test alongside competitor probe-based miRNA qPCR system). This eliminates false positives and ensures only robust and reliable miRNA signals.
The easy-to-follow, 3-hour protocol saves you both time and effort in the laboratory. Simply dilute the synthesized cDNA, combine it with the miRCURY LNA SYBR Green Master Mix and aliquot into the PCR plate, which is then ready to run in your real-time PCR instrument. The number of pipetting steps is reduced to a minimum, reducing technical variation. As a result, you can achieve extremely high reproducibility from day-to-day and even site-to-site (see figures Excellent day-to-day reproducibility and Excellent site-to-site correlation). High reproducibility is even possible when profiling difficult samples, such as RNA purified from serum (see figure Excellent reproducibility between RT reactions on total RNA from serum).