Custom Mix-n-Match Multi-Analyte ELISArray Kits

For the simultaneous detection of a custom panel of up to 12 cytokines or chemokines of your choice in multiple biological samples using an ELISA

Features

  • Detection of a custom panel of up to 12 cytokines or chemokines
  • High sensitivity and linearity
  • Screened and verified capture and detection antibodies
  • Uses standard ELISA equipment
Custom Mix-n-Match Multi-Analyte ELISArray Kit

Cat. No. / ID: 336111

1 plate (twelve 8-well strips) precoated with capture antibodies, and reagents
The Custom Mix-n-Match Multi-Analyte ELISArray Kits is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

This product will be discontinued as of November 12, 2020.

Custom Mix-n-Match Multi-Analyte ELISArray Kits allow you to choose up to 12 cytokines and chemokines for simple, simultaneous detection in multiple samples. You also have the choice to select fewer cytokines and increase the number of samples per plate. The optimized combination of high-quality capture and detection antibodies and colorimetric detection reagents provide each Custom Mix-n-Match ELISArray Kit with the best sensitivity and linearity available. Arranged in a 96-well plate, Custom Mix-n-Match ELISArray Kits use a standard ELISA protocol and provide the same quality and performance as the catalogued Multi-Analyte ELISArray Kits.

Choose cytokines and chemokines for your Custom Mix-n-Match Multi-Analyte ELISArray Kit from this excel file.

Performance

A Multi-Analyte ELISArray Kit was used to profile multiple cytokines secreted by stimulated human peripheral blood mononuclear cells (PBMCs). The time-dependent (0, 6, 18, 24, and 48 hours) patterns of 8 Th1/Th2 cytokine inductions by human PBMCs were montored in response to PMA (50 µg/ml) and ionomycin (1 µg/ml) (see figure " Patterns of Th1/Th2 cytokine induction"). The relative amount of each of the 8 cytokines was also profiled using the Multi-Analyte ELISArray Kit.

Large amounts of IL-2 and IFNγ, and to a lesser extent TNFα, are induced throughout, and apparently beyond, the time course tested. Only more moderate amounts of IL-4 and IL-5 are induced, reaching a maximum at approximately 24 hours of stimulation. The even lower levels of IL-10 and IL-13 that are secreted also reach a maximum at 24 hours and appear to decrease again at later times. IL-12 is not detectable after 48 hours of constant stimulation.

See figures

Principle

The Custom Mix-n-Match Multi-Analyte ELISArray Kit profiles a customized panel of up to 12 cytokines or chemokines in 6 cell supernatant (media), serum, or plasma samples using one simple protocol and one standardized development time (see figure " Multi-Analyte ELISArray plate layout"). Custom Mix-n-Match Multi-Analyte ELISArray Kits provide researchers with a rapid assessment of the level of multiple proteins of their choice (cytokines and chemokines) in multiple samples.

Commercially available capture and detection antibodies are screened to achieve the highest possible sensitivity and linearity for each cytokine or chemokine assay. All assays are optimized under uniform conditions to allow simultaneous detection without compromising performance (see figure " Optimized ELISA conditions enable the same development or incubation time").

See figures

Procedure

Custom Mix-n-Match Multi-Analyte ELISArray Kits use a standard sandwich ELISA protocol (see flowchart " ELISArray Protocol"). There is no requirement for special equipment; kits are compatible with a standard ELISA plate reader.

See figures

Applications

Custom Mix-n-Match Multi-Analyte ELISArray Kits are highly suited for research into autoimmune and immune disorders, cancer, immunology, infectious diseases, and many other areas of biological and medical research. They can be used in any application typically analyzed using individual ELISA kits.

Supporting data and figures

Resources

Kit Handbooks (2)
For the simultaneous detection of up to 12 cytokines or chemokines in multiple samples
For the simultaneous detection of up to 12 cytokines or chemokines in multiple samples

FAQ

What is the concentration of SureENTRY Transduction Reagent?
The concentration of SureENTRY Transduction Reagent is 10 mg/ml.
FAQ ID - 3708