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TopTaq Master Mix Kit

Read phase-out notice below
For highly reliable end-point PCR with unrivaled ease of use

  • Ready-to-use master mix with minimal pipetting steps
  • Fridge storage eliminates thawing time
  • Reliable high-yield PCR performance
  • Convenient room-temperature handling
  • Optimized protocol suitable for all PCR assays

The TopTaq Master Mix Kit offers maximum convenience by combining all the benefits of specific TopTaq DNA Polymerase with the advantage of a ready-to-use master mix. It contains TopTaq DNA Polymerase, the unique TopTaq PCR Buffer that minimizes the requirement for optimization, and dNTPs. The unique TopTaq Stabilizer included in the enzyme storage buffer allows storage of TopTaq Master Mix at 4°C and reaction setup at room temperature, without time-consuming thawing of reagents. Providing all components in a ready-to-use master mix reduces pipetting steps, which lowers the risk of contamination. High yields of PCR product are achieved, even after storing the TopTaq Master Mix for extended periods of time at 25°C or 4°C.


IMPORTANT NOTE: This product is phasing-out until October 15, 2020, and afterward, available only until stocks last. Visit the product page of the successor kit to view improved features or to request a trial kit.


For more information and FAQs on this transition, visit: www.qiagen.com/PCRresource.


Applications PCR, RT-PCR, DNA Fingerprinting
dNTP's included Yes (in Master Mix)
Enzyme activity 5' -> 3' exonuclease activity
Mastermix Yes
Reaction type PCR amplification
Real-time or endpoint Endpoint
Sample/target type Genomic DNA and cDNA
Single or multiplex Single
Storage conditions Fridge storage and room temperature setup
With/without hotstart Without Hotstart

Product Resources

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Kit Handbooks (1)
For standard and specialized end-point PCR applications without the need for optimization
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