AllPrep DNA/RNA Mini Kit

For simultaneous purification of DNA and RNA from cells and tissues
  • High-quality DNA and RNA from the same sample
  • Maximal yields of DNA and RNA from precious samples
  • Rapid purification with short, streamlined protocol
  • Ready-to-use DNA and RNA for any downstream analysis
The AllPrep DNA/RNA Mini Kit allows the simultaneous purification of genomic DNA and total RNA from the same biological sample. DNA is purified using the novel AllPrep DNA spin column. RNA is purified from the AllPrep column flow-through using an RNeasy Mini spin column. The kit can be automated on the QIAcube, and is compatible with up to 107 cells or 30 mg tissue.
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AllPrep DNA/RNA Mini Kit (50)
For 50 minipreps: AllPrep DNA Spin Columns, RNeasy Mini Spin Columns, Collection Tubes, RNase-Free Water and Buffers
 80204
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The AllPrep DNA/RNA Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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AllPrep DNA/RNA procedure.|Simultaneous purification of total RNA and genomic DNA.|Multiplex PCR of 8 targets.|Wide dynamic range.|High-quality total RNA.|
|Total RNA and genomic DNA were purified from 10 mg samples of rat liver using AllPrep technology (the AllPrep DNA/RNA Mini Kit). [A] Purified RNA samples were analyzed on a 1.2% formaldehyde agarose gel. [B] Purified DNA samples were analyzed on a 0.8% agarose gel. M: markers.|Genomic DNA was purified from mouse tissues using the AllPrep DNA/RNA Mini Kit. Multiplex PCR of 8 targets (192–1953 bp; GC content in the range of 19–61%) was performed using 100 ng DNA per sample. M: markers; NTC: no template control; Li: liver; K: kidney; Lu: lung.|Genomic DNA and total RNA were purified from 10-fold serial dilutions of rat kidney homogenate using the AllPrep DNA/RNA Micro Kit; at each dilution, DNA was purified from duplicates, and RNA was purified from 4 replicates. [A] Real-time PCR analysis of the Pgk1 gene and [B] real-time RT-PCR analysis of the Jun transcript showed reliable quantification over a wide dynamic range, from 5 mg down to 50 ng tissue.|Total RNA was purified from 1 x 106 Jurkat cells using the AllPrep DNA/RNA Mini Kit and analyzed on the Agilent 2100 Bioanalyzer. The RIN value was 10, indicating intact RNA. M: marker; 18S: 18S rRNA; 28S: 28S rRNA.|
Performance
A simple workflow allows the purification of high-quality DNA and RNA from the same sample. The purified genomic DNA has an average length of 15–30 kb and performs well in multiplex PCR (see figure "Multiplex PCR of 8 targets"). Total RNA is of high quality and has a RIN value of 10 indicating that the RNA is intact (see figure "Simultaneous purification of total RNA and genomic DNA" and figure "High-quality total RNA"). Analyses using genomic DNA and total RNA purified using AllPrep DNA/RNA technology show reliable quantification over a wide dynamic range (see figure "Wide dynamic range").
Principle

The AllPrep DNA/RNA Mini Kit is designed for purifying both genomic DNA and total RNA from a single cell or tissue sample. Since there is no need to divide the sample into two for separate purification procedures, maximum yields of DNA and RNA can be achieved. The purified DNA and RNA are eluted separately and ready to use in any downstream application.

Procedure

A simple workflow allows the purification of high-quality DNA and RNA from the same sample in as little as 35 minutes (see flowchart "AllPrep DNA/RNA procedure"). Both cultured cells and easy-to-lyse tissues can be processed. Genomic DNA is purified using the novel AllPrep DNA spin column, and total RNA is purified using an RNeasy Mini spin column. A supplementary protocol allows optional purification of protein. For experiments where DNA, RNA, and protein are regularly prepared from each sample, the AllPrep DNA/RNA/Protein Mini Kit can be used instead.

When disrupting and homogenizing tissues in Buffer RLT Plus (supplied with the AllPrep DNA/RNA Mini Kit), excessive foaming may occur. This foaming is substantially reduced by adding Reagent DX (supplied separately) to Buffer RLT Plus at a final concentration of 0.5% (v/v) before starting disruption and homogenization. Reagent DX has been carefully tested with the kit, and has no effect on RNA purity or on downstream applications such as real-time RT-PCR. Buffer RLT Plus containing Reagent DX can be stored at room temperature (15–25ºC) for at least 9 months.

Applications

The purified genomic DNA with an average length of 15–30 kb, depending on homogenization conditions, is suited for any application, including Southern-, dot-, and slot-blot analyses; and PCR and multiplex PCR.

The purified total RNA can be used in any application, such as: RT-PCR and real-time RT-PCR; differential display; cDNA synthesis; northern-, dot-, and slot-blot analyses; and microarrays.

A supplementary protocol allows optional purification of protein. The purified protein is denatured and can be used in applications such as SDS-PAGE, western blotting, and 2D gel electrophoresis.

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Feature
Specifications
Applications PCR, real-time PCR, microarray, blotting
Elution volume 100 µl
Format Spin column
Main sample type Cells, tissue
Processing Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA, total RNA
Sample amount 1 x 10e7/30 mg
Technology Silica technology
Time per run or per prep 35 minutes
Yield Varies

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Kit Handbooks
1
(EN) - AllPrep DNA/RNA Mini Handbook
For simultaneous purification of genomic DNA and total RNA from the same animal cell or tissue sample
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